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International Journal of Otolaryngology
Volume 2012 (2012), Article ID 962496, 6 pages
http://dx.doi.org/10.1155/2012/962496
Research Article

Measuring Thickness of Middle Ear Mucosa Using MRI and CT Imaging versus Histopathology

1Department of Otolaryngology and Head and Neck Surgery, Loma Linda University School of Medicine, Loma Linda, CA 92354, USA
2Otolaryngology Research, Jerry L. Pettis Memorial Veterans Administration Medical Center, Loma Linda, CA 92357, USA
3Mineral Metabolism Research, Jerry L. Pettis Memorial Veterans Administration Medical Center, Loma Linda, CA 92357, USA

Received 7 September 2011; Accepted 20 November 2011

Academic Editor: Joseph E. Kerschner

Copyright © 2012 Mary Ann Nyc et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. Otitis media (OM) is characterized by increased middle ear effusion and inflammation of middle ear tissue. In this study, we compared two radiographic methods of analyzing inflammation by measuring mucosal thickness (MT). Methods. 28 chinchillas were divided into three treatment groups consisting of a vehicle control group and two glucocorticoid groups. 6 underwent treatment by vehicle control, 10 were treated with ciprofloxacin 0.3%/dexamethasone 0.1% (DEX), and 10 received ciprofloxacin 0.2%/hydrocortisone 1% (HC). 96 hrs post-LPS inoculation, chinchillas were euthanized and their temporal bones were removed for analyses. Results. MRI scans ( 𝐹 = 1 4 6 . 0 8 6 1 , 𝑃 -value <0.0001) and histology ( 𝜒 2 = 4 0 . 5 2 6 7 , 𝑃 -value <0.0001) revealed statistically significant differences in MT measurements among treatment groups, whereas CT imaging did not. DEX-treated chinchillas exhibited overall significantly smaller MT values. Conclusion. Imaging MT was effective for determining severity of inflammation due to OM. Previous gold standard methods using histopathology compromise tissue integrity by chemical manipulation and dehydration effects. MRI and CT scanning are viable tools to preserve tissue and examine changes in MT. In this study, MRI provided more information about internal, soft tissue structures. In a clinical setting, MRI could be used for diagnosing and tracking severe or chronic OM.