More Than One Disease Process in Chronic Sinusitis Based on Mucin Fragmentation Patterns and Amino Acid Analysis
Table 3
Amino acid composition of papain digested chronic sinusitis mucins.
Amino acid
S1
S2
S3
S4
S5
S6
S7
S8
MUC5AC rich samples
MUC2 rich samples
% content of amino acids
% content of amino acids
His
2.3 ± 0.2
7.8 ± 0.5
7.4 ± 1.5
2.4 ± 0.3
5.2 ± 1.4
0.0 ± 0.0
3.6 ± 0.1
1.1 ± 0.1
Arg
9.8 ± 0.3
8.4 ± 0.4
6.2 ± 0.7
6.6 ± 2.6
4.1 ± 0.1
7.5 ± 0.1
3.9 ± 0.4
8.3 ± 0.6
Ser
26.2 ± 0.2
32.8 ± 2.8
34.9 ± 1.9
17.2 ± 2.7
14.3 ± 2.0
30.3 ± 0.6
19.8 ± 0.1
22.6 ± 1.6
Thr
17.1 ± 0.5
19.6 ± 1.5
30.3 ± 0.5
16.9 ± 0.9
18.0 ± 0.9
20.3 ± 0.9
12.5 ± 0.5
14.1 ± 1.3
Asp
4.8 ± 0.3
2.8 ± 0.1
4.6 ± 0.6
6.6 ± 1.1
4.9 ± 0.2
3.1 ± 0.1
2.5 ± 0.4
3.1 ± 0.1
Glu
3.9 ± 0.8
3.6 ± 0.3
12.8 ± 0.2
5.6 ± 0.7
9.5 ± 0.2
3.0 ± 0.6
5.6 ± 0.9
4.4 ± 0.5
Gly
00 ± 00
0.0 ± 00
3.4 ± 1.3
0.1 ± 0.1
7.2 ± 0.1
2.2 ± 0.3
7.3 ± 0.3
4.7 ± 0.2
Ala
27.7 ± 2.5
19.6 ± 0.6
22.4 ± 1.4
18.4 ± 3.2
3.6 ± 0.3
28.4 ± 0.3
14.8 ± 1.9
10.3 ± 1.3
Pro
23.1 ± 1.1
18.2 ± 0.3
20.7 ± 1.9
24.1 ± 4.1
5.8 ± 2.0
17.5 ± 2.1
13.2 ± 0.8
8.5 ± 0.1
Val
3.1 ± 0.1
4.2 ± 0.7
4.6 ± 0.1
6.0 ± 1.0
1.9 ± 0.4
2.8 ± 0.1
1.9 ± 0.1
5.6 ± 0.2
Ile/Leu/Phe
23.00 ± 1.5
16.2 ± 1.0
17.8 ± 2.8
12.6 ± 1.0
7.1 ± 0.7
11.4 ± 1.4
4.5 ± 0.2
7.8 ± 0.9
Lys
2.8 ± 0.5
7.0 ± 0.4
6.0 ± 2.4
3.6 ± 0.9
7.4 ± 0.2
0.1 ± 0.1
1.5 ± 0.4
2.5 ± 0.2
Calc. ptn
143.8 ± 2.2
140.2 ± 2.6
171.1 ± 6.9
120.1 ± 0.5
89.0 ± 0.2
126.6 ± 3.3
91.1 ± 2.7
93.0 ± 1.0
The values quoted are of the % amino acid contents in papain digested sinus mucins. The samples were arranged according to the proportions of the relevant predominant mucin. In the first 4 samples MUC5AC (S1–S4) was predominant and MUC2 was predominant in the other 4 samples (S5–S8). Amino acid content was calculated in quadruplicate samples and the mean content of amino acid in each sample was calculated. Calc. ptn: calculated total protein in ug/mg of freeze-dried mucin.
