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International Journal of Photoenergy
Volume 2008 (2008), Article ID 547301, 6 pages
UV-Activated Luminescence/Colourimetric O2 Indicator
1Department of Pure and Applied Chemistry, Westchem Graduate School of Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL, Scotland
2Chemical and Biological Sciences, Bell College of Technology, University of the West of Scotland, Hamilton ML3 0JB, Scotland
Received 26 July 2007; Accepted 6 November 2007
Academic Editor: Russell Howe
Copyright © 2008 Andrew Mills et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
An oxygen indicator is described, comprising nanoparticles of titania dispersed in hydroxyethyl cellulose (HEC) polymer film containing a sacrificial electron donor, glycerol, and the redox indicator, indigo-tetrasulfonate (ITS). The indicator is blue- coloured in the absence of UV light, however upon exposure to UV light it not only loses its colour but also luminesces, unless and until it is exposed to oxygen, whereupon its original colour is restored. The initial photobleaching spectral (absorbance and luminescence) response characteristics in air and in vacuum are described and discussed in terms of a simple reaction scheme involving UV activation of the titania photocatalyst particles, which are used to reduce the redox dye, ITS, to its leuco form, whilst simultaneously oxidising the glycerol to glyceraldehye. The response characteristics of the activated, that is, UV photobleached, form of the indicator to oxygen are also reported and the possible uses of such an indicator to measure ambient levels are discussed.
The presence of oxygen in food packaging usually has a detrimental effect on the food products contained therein. Oxygen does not only react chemically with food to cause oxidative rancidity, but moulds, growths, and aerobic microorganisms—which discolour and decompose food and make it offensive to smell and dangerous to eat—thrive in the presence of oxygen . Therefore, it is no surprise that the removal of oxygen in the food packaging industry is of immense importance. This is usually achieved via modified atmosphere packaging (MAP), a process in which the atmosphere within the food package is flushed and replaced with an inert gas, such as nitrogen or carbon dioxide, often combined with an efficient oxygen scavenger, resulting in an oxygen level of 0.1% or less within the food package [1, 2]. MAP increases the shelf life of many food products by a factor of 3-4 compared to that in air, making it a popular method of packaging food in the wholesale and retail food packaging industry.
There are many established methods for the detection of oxygen, which include the Clark electrode  and gas chromatography , however, such methods are too expensive and time consuming to allow 100% quality assurance. Consequently, there is an increasing interest in the development of cheap, easy-to-use oxygen indicators . This area of research has been dominated by the quenching of a polymer-encapsulated lumophore, such as ruthenium(II)-tris(4,7-diphenyl-1,10-phenanthroline), Ru(dpp, by oxygen. One of the few commercially-available products based on this approach is the OxySense system , whereby, Ru(dpp is encapsulated in silicone rubber dots, called xyDots™, which can be attached to the inside of a package or bottle. The xyDot is illuminated and the readily measured luminescence lifetime of the lumophore is equated to the oxygen level within the package. Unfortunately, the detection of oxygen using the OxySense system, or any optical sensor based on luminescence, requires the use of relatively expensive instrumentation for making the required lifetime or intensity measurements.
Unlike changes in luminescence intensity or lifetime, a sensor that changes colour in the presence of oxygen would be most desirable for MAP, given that the human eye can then act as the detector. Such indicators can take several forms, such as a tablet [7, 8] or a printed layer [9, 10]. This technology is typified by the Ageless Eye oxygen indicator, manufactured by the Mitsubishi Gas Company in Japan [7, 8, 11], that comprises a redox-indicator, usually methylene blue, which, in the absence of oxygen, is maintained in its colourless, chemically reduced, leuco form, by a reducing agent, such as glucose in an alkali medium. In the presence of oxygen leuco, methylene blue is oxidised to a highly coloured form. All components are mixed together, along with a nonredox dye, such as Acid Red 52, which provides a pink background colour, and pressed together to form a pellet, which is subsequently encapsulated in an oxygen-permeable, ion-impermeable plastic sachet to avoid any contact with the food. In the presence of oxygen, the Ageless Eye indicator changes from pink to purple in 2-3 hours, in a quasireversible process. However, this indicator needs to be stored and handled under anaerobic conditions. Such oxygen indicators are used in the food packaging industry, but mostly used as a research tool or fault diagnostic, since they have limited use elsewhere, because of cost and storage issues.
Lee et al. [12, 13] recently developed a new range of colourimetric oxygen indicators that are irreversible, reusable, and UV-light activated. Such “intelligent ink” oxygen sensors comprise a UV-absorbing semiconductor, such as Ti, a redox-indicator, such as methylene blue, a sacrificial electron donor, such as triethanolamine, and an encapsulating polymer such as hydroxyethyl cellulose; the ingredients are mixed together, with water as the solvent, to form an ink. The ink can be coated or printed subsequently onto a variety of substrates to produce a blue oxygen indicator film, which, when activated by UV light, becomes colourless. The activated, that is, UV-photobleached, film remains colourless unless, or until, exposed to oxygen, at which point the reduced methylene blue is reoxidised back to its original blue form.
The basic working principles, by which such an irreversible oxygen indicator works, are illustrated in Figure 1. Thus, upon UVA irradiation, ultraband gap illumination (hν) of the Ti semiconductor particles create electron-hole pairs, Ti(e−,h+). The photogenerated holes, h+, oxidise the mild sacrificial electron donor (SED) present, glycerol in this case, in the ink film and the remaining photogenerated electrons, that is, e− or Ti, as in Figure 1, reduce the redox-sensitive dye, to a reduced form, , which has a different colour to . In an ink film, the above key components are encapsulated in a polymer, such as hydroxyethyl cellulose (HEC) that is soluble in a common solvent (usually water). Thus, UV irradiation causes an -sensitive ink film to change colour (step 1, Figure 1). In the absence of oxygen, the photobleached dye will stay in this reduced—usually colourless—state indefinitely. However, upon exposure to oxygen, it is reoxidised to its original, highly coloured form (step 2, Figure 1).
In this paper, we describe a very oxygen sensitive version of this type of UV-activated indicator, in which the sacrificial electron donor is glycerol and the redox dye is indigo-tetrasulfonate (ITS). The latter is highly coloured and nonluminescent in its oxidised state but virtually colourless, highly luminescent, and very oxygen-sensitive in its reduced state, leuco indigo-tetrasulfonate (leuco-ITS).
Unless stated otherwise, all chemicals were purchased from Aldrich Chemical Company (Gillingham, Dorset, UK). The semiconductor, titanium dioxide (Ti), was P25 provided by Degussa (Frankfurt, Germany) and comprised particles ca. 30 nm in diameter, with an 80 : 20 anatase : rutile crystal phase composition.
2.2. Preparation of Indigo-Tetrasulfonate (ITS) Based Films
A typical example of an UV-activated luminescence/colourimetric oxygen-sensitive, ITS-based indicator ink, used to make the indicator films reported in this work, was prepared by adding 200 mg of glycerol to 2 g of a 5% wt hydroxyethyl cellulose (HEC) aqueous solution, to which 20 mg of P25 Ti and 5 mg of the redox indicator indigotetrasulfonate (ITS) had been added. The resulting mixture was stirred magnetically for 15 minutes, followed by 15 minutes sonication to disperse the usually aggregated titania particles, followed by a further 15 minutes stirring. Typically, an oxygen indicator film was prepared by placing 3-4 drops (ca. 0.4 ml) of this ink onto a cut-glass microscope slide ( cm), which was subsequently spun at 2500 rpm for 15 seconds. The resulting blue, transparent film was allowed to dry in the dark for 30 minutes before use.
All UV/V is spectra and absorbance versus time profiles were recorded using a Cary 50 BioVarian spectrophotometer. Luminescence spectra and luminescence intensity versus time profiles were recorded using a PerkinElmer LS50 fluorimeter and lifetime measurements were made with an IBH Fluorocube time-correlated single-photon counting system, using a NanoLed-03 source, which has its excitation peak wavelength at 370 nm. All UV irradiations were conducted using a W BLB handheld UVA light source (typical irradiance = 4 mW cm−2).
3. Results and Discussion
3.1. Chemical Reduction of an ITS Solution
An aqueous solution of leuco-ITS can be readily prepared from an ITS solution via the addition of a reductant, such as zinc amalgam. However, leuco-ITS is very oxygen-sensitive and readily oxidized back to ITS by ambient oxygen and so, in order to prevent this re-oxidation step occurring whilst recording the spectral properties of the reduced redox dye in solution, any oxygen needs to be removed. Thus, in a typical experiment, a dilute solution of ITS (), at pH4, was prepared and the absorbance spectra recorded, as illustrated in Figure 2 (broken line). ITS absorbs in the blue region of the visible spectrum, with a wavelength of maximum absorbance, , at 590 nm, which is typical for this dye . This solution was then deoxygenated via 5 cycles of a freeze-thaw process. Addition of a zinc-amalgam pellet to the deoxygenated ITS solution reduced the highly coloured ITS solution to its very pale yellow leuco-ITS form, as shown by the decrease in absorbance at 590 nm and the increase in absorbance at 385 nm in Figure 2 (spectra were recorded every 30 minutes). Excitation of leuco-ITS at this wavelength (385 nm) revealed a blue luminescence with an emission maximum at 485 nm. The uncorrected excitation ( nm) and emission spectrum ( nm) of the leuco-ITS aqueous solution are illustrated in the insert diagram of Figure 2. Single-photon counting revealed the lifetime of luminescence of leuco-ITS in aqueous solution, at pH4, to be microseconds.
3.2. Photobleaching of an ITS Oxygen Indicator Film
A parallel study, to that above, was carried out on a typical ITS oxygen indicator film, with one set of experiments carried out under vacuum ( mbar), that is, free, and the other under ambient conditions, that is, 21% . A typical absorbance spectrum of a blue-coloured indicator film under vacuum before (broken line) and after activation with UVA light is illustrated in Figure 3(a). From these results it can be seen that the initially blue-coloured ITS film is activated, that is, converted from ITS to leuco-ITS via the photoreduction of ITS by the UV-excited titania particles (see Figure 1) in under 3 minutes of UVA irradiation. This photoreduction process (step 1, Figure 1) produces a fall in absorbance of the film at 600 nm as a function of UVA irradiation time as indicated by the data in Figure 3(a) insert diagram. In contrast, under otherwise the same conditions, a typical oxygen indicator in an ambient environment takes twice as long to activate using the same UVA light source (I = 4 mW cm−2). The lower rate of photobleaching in the latter system is due to the presence of oxygen in the ambient environment which is able to reoxidise the reduced form of the dye via a dark reaction, that is step 2 in Figure 1.
Figure 3(b) contains photographs of a typical Ti/ITS/glycerol/HEC ink spun coated onto a coverslip (i) before and (ii) after photobleaching by UV irradiation (30 seconds, I = 7 mW cm−2) in air. Photograph (iii), in Figure 3(b), is after ca. 15 minutes in the dark under ambient conditions, during which time the ITS film regains its original colour (and loses its luminescence), due to the reoxidation of leuco-ITS to ITS by oxygen. Photographs (i)–(v) confirm that the ITS indicator films can be photoactivated under ambient conditions to produce a luminescent yellow, leuco-ITS film that is oxygen-sensitive.
The UV activation of a typical ITS oxygen indicator film can also be monitored by luminescence, given that leuco-ITS luminesces at 470 nm in the film (see Figure 3(b), photographs (iv) and (v)). The observed change in the emission spectrum of an ITS oxygen indicator film, under vacuum, upon exposure to UVA light as a function of time is illustrated in Figure 4. The insert diagram illustrates a plot of the variation in a film’s luminescence intensity as a function of UVA irradiation time, and, as in the absorption spectral changes in Figure 2, shows that in the absence of oxygen the film is activated in less than 3 minutes, in comparison to an oxygen indicator film in an ambient environment, which takes ca. 6 minutes due to the dark back reaction, that is, step 2 in Figure 1.
3.3. Dark Oxidation of an Activated ITS Film
Once activated, that is, photobleached, via step 1 in the reaction scheme in Figure 1, the stability of the photobleached oxygen indicator film depends on the level of oxygen present in the environment in which it finds itself. Thus, in the dark and in the absence of oxygen, the UV-activated oxygen indicator remains bleached indefinitely, whereas in the presence of oxygen, its colour is restored within seconds and the luminescence associated with the reduced form of ITS is quenched. This is nicely illustrated by the data in Figures 5 and 6 (broken lines), which depict the recorded change in absorbance at 600 nm and luminescence intensity at 470 nm, respectively, as a function of time, of an ITS-based oxygen indicator film that has been UV irradiated in a vacuum and subsequently exposed to an ambient atmosphere by opening up the system to air. In contrast to this data, the solid lines in Figures 5 and 6 correspond to the recovery of an UV-activated oxygen indicator film, irradiated under ambient conditions (i.e., 21% ) and maintained in this environment once the photobleaching process was stopped. Not surprisingly, the oxygen indicator film in an evacuated environment recovered its original blue colour when exposed subsequently to oxygen in the dark. However, the ITS indicator film which was initially photobleached in air recovered only ca. 50% of its original colour, possibly due to some photodegradation of the redox dye, presumably caused by the longer exposure to UVA light in air needed to photobleach the film, which probably promotes dye degradation via singlet oxygen production.
The insert diagrams in Figures 5 and 6 show that the dark process (step 2, Figure 1) gives a good fit to first-order kinetics for a UV-activated oxygen indicator film when photobleached and left to recover in an ambient atmosphere, or when photobleached in a vacuum and subsequently exposed to air. Such first-order kinetics are typical for UV-activated oxygen film indicators and provide support for the proposed scheme, that the kinetics of step 2 in the reaction scheme in Figure 1 depend directly upon the rate of diffusion of through the film. Other work indicates that the rate of this dark recovery process is first order with respect to the partial pressure of oxygen in the ambient atmosphere as with similar UV-activated, oxygen-sensitive film indicators. The first-order rate constants for the recovery of a typical UV-activated oxygen indicator film in an ambient environment and in a vacuum are given in Table 1. It remains unclear why the kinetics of step 2, the dye recovery step, are slower for an ITS film irradiated in air, compared to that for films irradiated in a vacuum, although an appreciable degree of dye degradation is observed for the latter process (vide supra). The above results stress the need for oxygen-free conditions when UV-activating these ITS-based indicators.
A novel, fast acting luminescent and colourimetric oxygen indicator ink is described, containing a sacrificial electron donor (glycerol) and the redox indicator (indigotetrasulfonate). The indicator is blue coloured in the absence of UV light, however, upon exposure to UV light it not only loses its colour but also luminesces, unless and until it is exposed to oxygen, whereupon, under dark conditions, its colour is restored. The initial photobleaching spectral (absorbance and luminescence) response characteristics in air and in vacuum are described and discussed in terms of a simple reaction scheme involving UV activation of the titania photocatalyst particles, which are used to reduce the redox dye, ITS, to its leuco form. The response characteristics of the activated, that is, UV-photobleached form of the indicator, to oxygen are also reported. This indicator appears more sensitive towards oxygen than previous UV-activated indicators, based on methylene blue, probably due to the lower redox potential  of ITS (−0.046 V versus SHE) compared to that of methylene blue (+0.028 V versus SHE) at pH7. This indicator appears susceptible to appreciable photodegradation when UV-activated under ambient (21% ) conditions. In the absence of oxygen, however, UV activation is not only more rapid, but also does not produce any significant photodegradation. Thus, the ink appears particularly suited for use in systems that are usually oxygen free, before UV activation, as a means of indicating any subsequent leak or tampering.
The authors are pleased to acknowledge a grant to C. Tommons from Bell College Research Grants Committee and M. C. Tedford thanks the Royal Society of Chemistry Research Fund.
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