Manipulation of Energy Transfer Processes in Nanochannels
Figure 3
FRET case study on a mixed Py,Ox-zeolite L composite. (a) Excitation and fluorescence spectra of both dyes in zeolite L scaled to the same height at the maximum. The different overlap areas are indicated by grey shading. (b) Middle: photographic image of dye-loaded zeolite L layers upon monochromatic irradiation at 485 nm and observation through a 500 nm cut-off filter. The two samples indicated as P and Ox are references loaded with pure Py and Ox, respectively. Samples A to E contain a 1:1 mixture of Py and Ox with the following loading for each dye: A, ; B, ; C, ; D, E, . Left: Crystal and donor-acceptor pair describing the situation in sample A. Right: Crystal and donor-acceptor pair describing the situation in sample E [63]. (c) Fluorescence microscopy pictures, visualizing the diffusion of Ox to Py in zeolite L, taken after 20 minutes (), 60 minutes (), 470 minutes (3a, 3b), and 162 hours (), respectively. Py was specifically excited, with the exception of 3b, where Ox was specifically excited. Two crystals of each image are framed. The scale given in 1 corresponds to a length of 1.5 m [64].
