Pharmacogenomics in Children: Advantages and Challenges of Next Generation Sequencing Applications

Figure 1

Schematic representation of the principles of massive parallel sequencing using beads. Double-stranded DNA (a) is fragmented into single-stranded DNA (b) which is subsequently coupled, via adaptors, to agarose beads by oligonucleotides complementary to the adaptor (c). These beads are submerged in an emulsion where amplification of the single DNA fragment occurs (d). Subsequently, the bead is placed into a well (e), already equipped with all reagents for sequencing (small beads). Within these wells, parallel sequencing of the different DNA fragments occurs, resulting in the generation of the genetic code of the fragment (f).