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Figure 2: Detailed immunohistochemical representation of MIF-1 induced brain activation by c-Fos immunoreactivity. The panels on the left show basal c-Fos activity in different regions. The second panels show c-Fos immunoreactivity 4 hours after iv injection of MIF-1 (10  𝜇 g/mouse). The third panels show c-Fos immunoreactivity 4 hours after icv injection of MIF-1 (1  𝜇 g/mouse). The last panels show NeuN (+) neurons (red), DAPI-labeled nucleus (blue), as well as c-Fos (green) in sections after iv injection. (a) Cingulate cortex, (b) Thalamic nucleus, (c) Paraventricular thalamic nucleus, anterior part, (d) Paraventricular thalamic nucleus, posterior part, (e) Infralimbic cortex, (f) Paraventricular nucleus of the hypothalamus, (g) medial amygdaloid nucleus, (h) Fiber tract in piriform cortex, and (i) Nucleus accumbens.