MIF-1 activates cultured SH-SY5Y neurons, shown by western blotting of c-Fos. (a) MIF-1 increased neuronal c-Fos at 1, 2, and 3 hours at a concentration of 10 ng/mL, showing its time-dependent pattern. (b) MIF-1 increased c-Fos at all concentrations tested at 1 hour, showing its dose-response.
