The Effects of Ghrelin on Energy Balance and Psychomotor Activity in a Goldfish Model: An Overview
Figure 3
Effect of ghrelin on locomotor activity in goldfish. Panel (a) shows the effect of a single ICV injection of ghrelin on locomotor activity. Panel (b) shows the effect of IP-injected ghrelin on locomotor activity. The open field test, which started 15 min after ICV or IP injection and lasted 45 min, was performed with a video tracking system for automatic recording of goldfish behavior (EthoVision Pro, Noldus Information Technology, Wageningen, Netherlands). Each column and bar represent the mean and S.E.M., respectively, and the numbers in parentheses in the legend indicate the number of fish used in each group. The significance of differences at each time point, compared to the vehicle-injected group, was evaluated by one-way ANOVA with Bonferroni’s method (*, **).
