Easy and Rapid Purification of Highly Active Nisin
Figure 5
IC50 determination of the nisin purification fractions. Growth inhibition experiments were performed with nisin obtained from the different elution fractions of the purifications of commercial nisin (a) and of nisin secreted by the L. lactis NZ9700 strain (b). The log of the used nisin concentration of each elution fraction is plotted against the normalized optical density of L. lactis NZ9000 after five hours of growth. Shown are the inhibition curves for the NaCl elution fractions of 200 mM (o), 400 mM (∆), 600 mM (∇), 800 mM (◊), and 1 M (*). Data was fitted and evaluated according to (1).
