In Vitro Selection of Cathepsin E-Activity-Enhancing Peptide Aptamers at Neutral pH
Figure 2
Surveying the building block peptides used for the secondary library. (a) Cluster analysis of the peptides selected from the primary library, using ClustalW supported by DDBJ (DNA Data Bank of Japan). Consensus sequences are shown in bold. (b) Schematic drawing of 23 tetramer peptide blocks used for the secondary library construction by the ASAC (all-steps-all-combinations) method. In addition to the blocks from the selected sequences (which also contain the SF-link construct), arbitrarily chosen blocks of (GGGG)/n were combined.
