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Figure 5: 2-DE gel image of GRp58 phosphorylation pattern of rat gastric from fed, 48 h fasted rats, and 48 h fasted rats treated with leptin and sacrificed after 6 h and 24 h of leptin administration. GRp58 protein acidic isoelectric points decreased in 2DE gels in fasted and leptin treated rats compared to vehicle-treated control fed rats. Gastric mucosa proteins were extracted and separated on an immobilized pH 4–7 non-linear gradient strip followed by separation on a 12% polyacrylamide gel. Red arrows show the different phosphorilation pattern observed in different treatments.