Table 3: Identification of GRp58 protein in the gastric mucosa of the rat. Rat gastric proteins were separated by 2-DE and identified by means of MALDI-TOF. The protein identified represents the spots shown in Figures 1(a) and 1(b): protein disulfide-isomerase A3 precursor (Disulfide isomerase ER-60; ERp60; 58 kDa microsomal protein; p58; ERp57; HIP-70; Q-2; 35 petides), the spots labelled as P1′ and P2′ are the same spots as P1 and P2 but in different gels.

Protein MW: 57043.9  Protein PI: 5.88  Accession No: gi | 1 3 5 2 3 8 4 |

Observed Mr (expt)Mr (calc)± da± ppmStart-endSequenceSpot number

823.4785823.45590.022627190–196IVAYTEKp1′,p1
866.4574866.4617−0.00435387–393YKELGEKp1′,p1
877.4915877.4892750.00253268–275LNFAVASRp1′,p1
995.5628995.5632−0.00040102–111QAGPASVPLRp1′,p1
997.5101997.459−0.0511−51153–161DASVVGFFRp2
1084.56741084.56880.0014195–104YGVSGYPTLKp2′,p1,p2,P3
1123.65821123.65890.00071101–111KQAGPASVPLRp1′,p1
1125.5471125.54360.00343243–251NTKGSNYWRp1
1172.54041172.4702−0.0702−60336–344FVMQEEFSRp1,p2
1179.58651179.62390.037432174–183AASNLRDNYRp2′,p1,p2
1188.53541188.5305−0.0049−4344–336FVMQEEFSRp2′,p1,p2,P3
1191.60051191.60840.0079763–73LAPEYEAAATRp1′,p2′,p1,p2,P3,P4
1236.51271236.5029−0.0098−8108–119DGEEAGAYDGPRp2′,p1,p2,P3
1244.66331244.6276−0.0357−29184–194FAHTNVESLVKp1,P3
1341.68371341.683−0.0007−1449–460GFPTIYFSPANKp2′,p1,p2,P3,P4
1347.70431347.70150.0028233–44RLAPEYEAAATRp1
1373.67361373.5839−0.0897−65352–362FLQEYFDGNLKp2
1394.65871394.5729−0.0858−62162–173DLFSDGHSEFLKp1,p2
1396.69541396.6727−0.0227−16367–379SEPIPETNEGPVKp2′,p1,p2,P3,P4
1397.57841397.70630.12799283–94VDCTANTNTCNKp1′,p2′,p2,P3,P4
1397.70591397.70630.00040472–482ELNDFISYLQRp1′,p2′,p1,P3,P4
1469.77871469.691−0.0877−60449–461GFPTIYFSPANKKp1,p2,P4
1472.68381472.6184−0.0654−44336–347FVMQEEFSRDGKp2
1488.67871488.6698−0.0089−6336–347FVMQEEFSRDGKP3
1529.77471529.7750.00030352–363FLQEYFDGNLKRp1′,p2′,p1,p2,P3,P4
1593.84831593.85510.00684483–496EATNPPIIQEEKPKp2′,p1,p2,P3,P4
1607.74761607.7640.016410259–271DLLTAYYDVDYEKp1′,p2′,p2,P3,P4
1636.75231636.6552−0.0971−59434–448MDATANDVPSPYEVKp1,p2,P4
1652.74721652.6615−0.0857−52434–448MDATANDVPSPYEVKp2
1652.76621652.77040.00423105–119IFRDGEEAGAYDGPRp1′,p2′,p1,p2,P3,P4
1744.88641744.7698−0.1166−67131–146QAGPASVPLRTEDEFKp2
1746.92861746.8311−0.0975−56289–304TFLDAGHKLNFAVASRp1′
1800.93771800.9132−0.0245−14364–379YLKSEPIPETNEGPVKp1′,p2′,p1,p2,P3,P4
1950.93311950.7816−0.1515−78259–274DLLTAYYDVDYEKNTKp2
2463.12792462.9673−0.1606−6583–104VDCTANTNTCNKYGVSGYPTLKp2