Molecular Cloning and Sequence Analysis of the cDNAs Encoding Toxin-Like Peptides from the Venom Glands of Tarantula Grammostola rosea
Figure 1
Homology analysis of ICK motif toxins. (a) Homology alignment of ICKs. The putative signal sequences deduced by SignalP 3.0 server (http://www.cbs.dtu.dk/services/SignalP/) are indicated by dotted box. Signal sequences and prepro-sequences of GTx1-6, GTx1-24, GTx2-4, GTx2-5, HnTx-IV, PaurTx3, TLTx1, CcoTx2, and VSTx1, 2, 3 are not determined. Mature toxin regions are indicated by closed box. Conserved cysteine residues are indicated by closed boxes filled with gray color. (b) Phylogenetic tree of ICK toxins. Amino acid alignment and phylogenetic tree construction were performed using the MegAlign program by Clustal W and neighbor-joining method (DNASTAR, Madison, USA) based on the alignment (a) of mature peptides. A scale below the tree indicates the number of amino acid substitutions per 100 residues for protein sequences. Known molecular targets are indicated following to the peptide names. Accession numbers: GTx1 and GTx2 families, AB200996-AB201024 and AB671302-AB671308; HaTx1, AB200991; HaTx2, AB200992; VSTx1, AB200994; GsAFI, AB200995; GsAFII, AB612242; GrTx1, AB671300 and AB671301; ω-GrTx SIA, AB612243; GsMTx4, AB201020; PaurTx3, P84510; CcoTx2, P84508; TLTx1, P83745; VSTx2, P0C2P4; VSTx3, P0C2P5.
