Identification and Characterization of a Novel Nontranslated Sequence Variant of the Human Intestinal Di-/Tripeptide Transporter, hPEPT1
Figure 1
Molecular characterization of human PEPT1-RFI; a novel sequence variant. (a) Nucleotide sequence of human PEPT1-RFI cDNA from the 5′untranslated region to the deduced hPEPT1-RFI stop codon. The insertions 113Tins and 693Tins (marked in bold) and one deletion (848Tdel) (marked by a line) separate this sequence from the hPEPT1-RF sequence. Deduced start and stop codons are underlined. (b) Putative amino acid composition of hPEPT1-RFI [accession number AL353574.8]. (c) Topographic orientation of the hPEPT1-RFI protein as predicted by the HMMTOP transmembrane topology prediction server [6, 7]. The hPEPT1-RFI corresponds to hPEPT1 in the sequence shown in black circles. The image was generated using TOPO2, transmembrane protein display software (Johns S. J., http://www.sacs.ucsf.edu/TOPO2/).
