Identification and Characterization of a Novel Nontranslated Sequence Variant of the Human Intestinal Di-/Tripeptide Transporter, hPEPT1
Figure 4
In vitro translation of hPEPT1 and TRI-Xef, but absence of hPEPT1-RFI protein. Equal amounts of plasmids with inserts of hPEPT1-RFI, hPEPT1, TRI-Xef. and empty plasmid were transcribed in vitro. Of each transcription mix, 2 μl were used for the in vitro translation mixed with reticulocyte lysate. PAGEr Tris glycine gels were exposed to a phosphor imager screen overnight for visualization of the protein-incorporated []-Methionine. No protein band was identified in the gel after in vitro translation of the hPEPT1-RFI containing plasmid (lane 2). Protein bands are shown in the positive controls (lanes 1 and 3) at the expected molecular weights. As expected, negative controls showed no protein bands (lanes 4 and 5).
