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International Journal of Peptides
Volume 2012 (2012), Article ID 745027, 6 pages
http://dx.doi.org/10.1155/2012/745027
Research Article

A Novel Cellular Model to Study Angiotensin II AT2 Receptor Function in Breast Cancer Cells

1Inserm, U1016, Institut Cochin, Paris, France
2CNRS, UMR 8104, Paris, France
3University Paris Descartes, Paris, France
4Department of Biochemistry and Immunology, Faculty of Medicine at Ribeirao Preto, University of São Paulo, 14049-900 Ribeirao Preto, SP, Brazil

Received 29 June 2011; Revised 6 September 2011; Accepted 6 September 2011

Academic Editor: Jean-Marie Zajac

Copyright © 2012 Sylvie Rodrigues-Ferreira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Recent studies have highlighted the AT1 receptor as a potential therapeutic target in breast cancer, while the role of the AT2 subtype in this disease has remained largely neglected. The present study describes the generation and characterization of a new cellular model of human invasive breast cancer cells (D3H2LN-AT2) stably expressing high levels of Flag-tagged human AT2 receptor (Flag-hAT2). These cells exhibit high-affinity binding sites for AngII, and total binding can be displaced by the AT2-selective antagonist PD123319 but not by the AT1-selective antagonist losartan. Of interest, high levels of expression of luciferase and green fluorescent protein make these cells suitable for bioluminescence and fluorescence studies in vitro and in vivo. We provide here a novel tool to investigate the AT2 receptor functions in breast cancer cells, independently of AT1 receptor activation.