Research Article
Development of the Schedule for Multiple Parallel “Difficult” Peptide Synthesis on Pins
Table 2
Peptides synthesized by the modification (1) of the standard parallel solid-phase peptide synthesis schedule on pins.
| Peptide number | Peptide sequence | HCV protein source of octapeptide fragment | Calculated molecular mass of peptide, | Masses of molecular ions in MALDI-TOF mass spectra, |
| 1 | Biotin-S GPGCVPCVR-(KP) | E1 | 1551.9 | 1551.8 | 2 | Biotin-SGSGGCVPCVRE-(KP) | E1 | 1583.9 | 1583.8 | 3 | Biotin-SGSGYVGDLCGS-(KP) | E1 | 1534.8 | 1556.3 (+Na+) | 4 | Biotin-SGSGVGDLCGSV-(KP) | E1 | 1470.7 | 1492.7 (+Na+) | 5 | Biotin-SGSGGDLCGSVF-(KP) | E1 | 1518.8 | 1540.7 (+Na+) | 6 | Biotin-SGSGDLCGSVFL-(KP) | E1 | 1574.9 | 1596.8 (+Na+) | 7 | Biotin-SGSGQLFTFSPR-(KP) | E1 | 1717.0 | 1716.9 | 8 | Biotin-SGSGQDCNCSIY-(KP) | E1 | 1666.9 | 1688.7 (+Na+) | 9 | Biotin-SGSGCNCSIYPG-(KP) | E1 | 1577.8 | 1599.6 (+Na+) | 10 | Biotin-SGNCSIYPGH-(KP) | E1 | 1611.9 | 1611.3 1633.3 (+Na+) | 11 | Biotin-SGSGAWDMMMNW-(KP) | E1 | 1806.2 | 1827.7 (+Na+) | 12 | Biotin-SGSGWDMMMNWS-(KP) | E1 | 1822.2 | 1843.7 (+Na+) | 13 | Biotin-SGSGDMMMNWSP-(KP) | E1 | 1733.1 | 1770.7 (+Na+; +O) 1754.7 (+Na+) 1786.6 (+Na+; +2O) | 14 | Biotin-SGSGMMMNWSPT-KPP | E1 | 1719.1 | 1756.7 (+Na+; +O) 1772.7 (+Na+; +2O) 1740.7 (+Na+) 1788.7 (+Na+; +3O) | 15 | Biotin-SGSGAGAHWGVL-(KP) | E1 | 1531.8 | 1553.8 (+Na+) 1531.8 | 16 | Biotin-SGSGGAHWGVLA-(KP) | E1 | 1531.8 | 1553.8 (+Na+) 1531.8 | 17 | Biotin-SGSGAHWGVLAG-(KP) | E1 | 1531.8 | 1553.8 (+Na+) 1531.8 | 18 | Biotin-SGSGSMVGNWAK-(KP) | E1 | 1613.9 | 1635.8 (+Na+) 1613.8 | 19 | Biotin-SGSGMVGNWAKV-(KP) | E1 | 1625.9 | 1625.8 1647.8 (+Na+) 1641.8 (+O) 1663.8 (+Na+; +O) | 20 | Biotin-SGSGVGNWAKVL-(KP) | E1 | 1607.9 | 1629.9 (+Na+) 1607.9 | 21 | Biotin-SGSGINTNGSWH-(KP) | E2 | 1649.8 | 1671.7 (+Na+) 1649.7 | 22 | Biotin-SGSGNTNGSWHI-(KP) | E2 | 1649.8 | 1649.8 1671.8 (+Na+) | 23 | Biotin-SGSGTNGSWHIN-(KP) | E2 | 1649.8 | 1649.8 1671.8 (+Na+) | 24 | Biotin-SGSGNGSWHINR-(KP) | E2 | 1704.9 | 1704.8 | 25 | Biotin-SGSGALNCNDSL-(KP) | E2 | 1570.8 | 1592.7 (+Na+) | 26 | Biotin-SGSGPVVVGTTD-(KP) | E2 | 1508.8 | 1530.8 (+Na+) | 27 | Biotin-SGSGVVVGTTDR-(KP) | E2 | 1567.8 | 1567.8 | 28 | Biotin-SGSGWGENETDV-(KP) | E2 | 1670.8 | 1692.8 (+Na+) | 29 | Biotin-SGSGGNWFGCTW-(KP) | E2 | 1691.9 | 1709.8 (+H2O) | 30 | Biotin-SGSGNWFGCTWM-(KP) | E2 | 1766.1 | 1787.8 (+Na+) | 31 | Biotin-SGSGFGCTWMNS-(KP) | E2 | 1666.9 | 1688.7 (+Na+) | 32 | Biotin-SGSGKCGSGPWL-(KP) | E2 | 1535.8 | 1557.7 (+Na+) 1535.7 | 33 | Biotin-SGSGTGFTKTCG-(KP) | E2 | 1568.9 | 1568.8 | 34 | Biotin-SGSGCGSGPWLT-(KP) | E2 | 1541.8 | 1563.7 (+Na+) | 35 | Biotin-SGSGGSGPWLTP-(KP) | E2 | 1535.8 | 1557.7 (+Na+) | 36 | Biotin-SGSGSGPWLTPR-(KP) | E2 | 1634.9 | 1634.8 | 37 | Biotin-SGSGGPWLTPRC-(KP) | E2 | 1651.0 | 1650.9 | 38 | Biotin-SGSGHYPCTVNF-(KP) | E2 | 1702.0 | 1701.8 1723.8 (+Na+) | 39 | Biotin-SGSGRMYVGGVE-(KP) | E2 | 1631.9 | 1631.9 1647.8 (+O) | 40 | Biotin-SGSGTGFTKTCG-(KP) | E2 | 1612.9 | 1612.8 1634.8 (+Na+) | 41 | Biotin-SGSGYVGGVEHR-(KP) | E2 | 1637.9 | 1637.9 1659.8 (+Na+) | 42 | Biotin-SGSGVGGVEHRL-(KP) | E2 | 1587.8 | 1587.8 | 43 | Biotin-SGSGAACNWTRG-(KP) | E2 | 1599.8 | 1599.8 | 44 | Biotin-SGSGACNWTRGE-(KP) | E2 | 1657.9 | 1657.7 | 45 | Biotin-SGSGCNWTRGER-(KP) | E2 | 1743.0 | 1742.8 | 46 | Biotin-SGSGNWTRGERC-(KP) | E2 | 1743.0 | 1742.8 | 47 | Biotin-SGSGLEDRDRSE-(KP) | E2 | 1740.0 | 1740.8 1762.8 (+Na+) | 48 | Biotin-SGSGEDRDRSEL-(KP) | E2 | 1740.0 | 1740.8 1762.8 (+Na+) | 49 | Biotin-SGSGDRDRSELS-(KP) | E2 | 1698.9 | 1698.7 | 50 | Biotin-SGSGRDRSELSP-(KP) | E2 | 1680.9 | 1680.8 | 51 | Biotin-SGSGDRSELSPL-(KP) | E2 | 1637.9 | 1637.8 1659.8 (+Na+) | 52 | Biotin-SGSGRSELSPLL-(KP) | E2 | 1636.0 | 1635.8 | 53 | Biotin-SGSGIHLHQNIV-(KP) | E2 | 1570.9 | 1592.7 (+Na+) | 54 | Biotin-SGSGTTLPALST-(KP) | E2 | 1524.8 | 1546.7 (+Na+) | 55 | Biotin-SGSGTLPALSTG-(KP) | E2 | 1480.7 | 1502.7 (+Na+) | 56 | Biotin-SGSGIHLHQNIV-(KP) | E2 | 1695.0 | 1694.8 1716.8 (+Na+) | 57 | Biotin-SGSGSDLPALST-(KP) | E2 | 1524.8 | 1546.7 (+Na+) | 58 | Biotin-SGSGTPMPALST-(KP) | E2 | 1538.9 | 1576.6 (+Na+; +O) | 59 | Biotin-SGSGDLPALSTG-(KP) | E2 | 1494.7 | 1516.6 (+Na+) | 60 | Biotin-SGSGPMPALSTG-(KP) | E2 | 1494.8 | 1532.6 (+Na+; +O) | 61 | Biotin-S GETLSVGGS-(KP) | E2 | 1470.7 | 1492.4 (+Na+) | 62 | Biotin-S GETIVTGGT-(KP) | E2 | 1498.7 | 1520.6 | 63 | Biotin-S GETAVSGGT-(KP) | E2 | 1442.6 | 1464.4 (+Na+) | 64 | Biotin-S GETRVSGGT-(KP) | E2 | 1527.7 | 1527.3 | 65 | Biotin-SGSGGTYTTGGA-(KP) | E2 | 1448.6 | 1470.6 (+Na+) | 66 | Biotin-SGSGTTYTTGGS-(KP) | E2 | 1508.7 | 1530.4 (+Na+) | 67 (1a) | Biotin-SGSGTTKVIGGT-(KP) | E2 | 1497.8 | 1497.6 | 68 | Biotin-SGSGGTRTMGGA-(KP) | E2 | 1471.7 | 1471.5 | 69 | Biotin-S SGGTHVTGGS-(KP) | E2 | 1436.6 | 1436.4 | 70 | Biotin-SGSGGTRVSGGT-(KP) | E2 | 1455.7 | 1455.4 | 71 | Biotin-SGS GSTHVTGGA-(KP) | E2 | 1450.6 | 1450.5 | 72 | Biotin-S GSTYTTGGS-(KP) | E2 | 1494.7 | 1516.3 (+Na+) | 73 | Biotin-S GSTTITGGS-(KP) | E2 | 1444.6 | 1466.5 (+Na+) | 74 | Biotin-SGSGSTRVTGGA-(KP) | E2 | 1469.7 | 1469.5 | 75 | Biotin-SGS GQTHTTGGS-(KP) | E2 | 1528.7 | 1528.8 1551.1 (+Na+) | 76 | Biotin-SGSGGTRVSGGT-(KP) | E2 | 1509.7 | 1509.4 1531.3 (+Na+) | 77 | Biotin-SGSGQTYVTGGA-(KP) | E2 | 1517.7 | 1539.4 (+Na+) | 78 | Biotin-SGSGKTYTTGGA-(KP) | E2 | 1519.7 | 1519.7 1541.7 (+Na+) | 79 | Biotin-S GKTHVTGGS-(KP) | E2 | 1507.7 | 1507.5 | 80 | Biotin-S RTHVTGGS-(KP) | E2 | 1535.7 | 1535.7 | 81 | Biotin-SGSGGTYVTGGA-(KP) | E2 | 1446.6 | 1535.7 | 82 | Biotin-GRTRLTGGN-(KP) | E2 | 1595.8 | 1595.6 | 83 | Biotin- RTKTIGGT-(KP) | E2 | 1554.8 | 1554.6 | 84 | Biotin-SGSGATYTTGGA-(KP) | E2 | 1462.7 | 1462.4 1484.5 (+Na+) | 85 | Biotin-SGSGATHVTGGT-(KP) | E2 | 1464.7 | 1464.3 | 86 | Biotin-S GNTYTTGGS-(KP) | E2 | 1521.7 | 1543.4 (+Na+) | 87 (3a) | Biotin-S GNTKLMGGT-(KP) | E2 | 1542.8 | 1542.5 | 88 | Biotin-S GNTRTTGGT-(KP) | E2 | 1528.7 | 1528.6 | 89 (4a) | Biotin-SGSGNNYVTGGA-(KP) | E2 | 1516.7 | 1538.3 (+Na+) | 90 | Biotin-SGSGDTHVTGGS-(KP) | E2 | 1494.6 | 1494.4 | 91 | Biotin-S GHTRTTGGA-(KP) | E2 | 1521.7 | 1521.4 | 92 (5a) | Biotin-SGSGDTRVVGGQ-(KP) | E2 | 1552.8 | 1552.6 | 93 | Biotin-SGHTYTTGGT-(KP) | E2 | 1558.7 | 1558.7 | 94 | Biotin-SGHTHTTGGV-(KP) | E2 | 1530.7 | 1530.4 | 95 | Biotin-SGHTHVTGGV-(KP) | E2 | 1528.7 | 1528.5 | 96 | Biotin-S GDTYTTGGS-(KP) | E2 | 1522.7 | 1544.4 (+Na+) |
|
|
Amino acid residues, for which repeated coupling was performed while using modification (A) of the standard procedure, are shown in bold.
|