International Journal of Plant Genomics
Volume 2007 (2007), Article ID 51421, 5 pages
doi:10.1155/2007/51421
Research Article

Generation and Screening of a BAC Library from a Diploid Potato Clone to Unravel Durable Late Blight Resistance on Linkage Group IV

Ingo Hein,1 Karen McLean,1 Boulos Chalhoub,2 and Glenn J. Bryan1

1Genetics Programme, SCRI, Invergowrie, Dundee DD2 5DA, UK
2UMR INRA-CNRS-UEVE de Recherches en Genomique Vegetale, 2 rue Gaston Cremieux, BP5708, Evry Cedex 91057, France

Received 18 April 2007; Revised 16 July 2007; Accepted 18 September 2007

Academic Editor: Andrew C. James

Copyright © 2007 Ingo Hein et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We describe the construction and screening of a large insert genomic library from the diploid potato clone HB171(13) that has been shown to express durable quantitative field resistance to Phytophthora infestans, the causal agent of potato late blight disease. Integrated genetic mapping of the field resistance quantitative trait locus with markers developed from populations segregating for Rpi-blb3, Rpi-abpt, R2, and R2-like resistance, all located on linkage group IV, has positioned the field resistance QTL within the proximity of this R gene cluster. The library has been successfully screened with resistance gene analogues (RGA) potentially linked to the R gene cluster. Over 30 positive BAC clones were identified and confirmed by PCR and Southern hybridisations to harbour RGA-like sequences. In addition, BAC end sequencing of positive clones has corroborated two BAC clones with a very high level of nucleotide similarity to the RGA probes utilised.