Review Article

Soybean Genomics: Developments through the Use of Cultivar “Forrest”

Figure 3

Genetic systems used with Forrest germplasm and the inbred soybean crop (a). The ancestry of Forrest and Hartwig showing the known cultivars that were crossed and the relationship between Flyer and Williams 82 (b). A diagram showing how NILs derived from RILs fix most loci but allow the continued segregation of heterozygous regions in inbred crops like soybean. The effect is to Mendelize a few of the loci contributing to QT while causing the majority to be fixed. A dark pod parent was crossed with a light colored pod parent; the F1 heterozygous type (shown as purple pods) was selfed; and F2 progeny was advanced to the F5 by selfing. A heterozygous plant at any time or heterogeneous RIL at or later identified is shown as purple pods. Single plants are extracted and seed increased. NILs that result may fix the heterogeneous region to the parent 1 allele, the parent 2 allele, or are still heterogeneous. Occasionally heterozygous plants are found within some heterogeneous NILs even at the and the progeny of such plants can be used to find new recombination events. Shown are the results with Satt309 and NIL11 plant 3 and eighteen of the progeny collected from it (adapted from [40]).
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