Soybean Genomics: Developments through the Use of Cultivar “Forrest”
Table 5
Progress in the soybean physical map builds 2 to 5.
Automated Build 2 Sept. 2001
Manual Build 3 Oct 2002
Manual Build 4 Oct 2003 Total
Judged by BACs/unique band to be (pploid) [unique]
Manual Build 5 Jan 2008
BAC clones in FPC database
81,024
83,026
78,001
78,001
BACs used in contig assembly
75,568
78,001
72,942
72,837
Number of singletons
5,884
4954
27,1812
17,942
Marker anchored singletons
0
0
120
63
Clone in contigs (fold genome)
69,684
73,069
45,135
58,765
Fold genome in contigs
8.7
9.1
5.6
62
Number of contigs
5,488
2,907
2,854
(646)[2208]
521
Anchoring Markers
0
385
404
(280)[124]
1,523
Anchored Contigs
0
781
742
(181)[223]
455
Contlgs contain: >25 clone
220
921
477
(268)[209]
335
10–25 clones
3,038
920
1,458
(433)[1025]
110
3–9 clones
1,845
850
820
(0)[820]
43
2 clones
385
216
99
(0)[99]
33
Unique bands in the
contigs
396,843
345,457
#258,240
(64,560)
257,356
Length of the contigs (Mb)
1,667*
1,451*
1.037
(0.258) [0.769]
1.034
*Based on 4.00 kbp per unique hand. # Based on 4.05 kbp per unique band, for 2,854 contigs containing 68 unique bands in 15 clones, 264 duplicated region contigs
containing 68 unique bands in 30 clones I5,840 unique bands and 406 highly repeated region contigs containing 68
unique bands is 60 clones, 48,720 unique bands.
