Soybean Genomics: Developments through the Use of Cultivar “Forrest”

Table 6

Summary of sequence coverage of the three minimum tile paths (MTPs) used for BAC end sequencing made from three BAC libraries. To calculate the percentage of the soybean genome covered by the clones (clone coverage) in our EcoRI-(MTP4E) and BamHI or HindIII insert libraries (MTP2BH and MTP4BH), the genome size of soybean was assumed to be 1130 Mb. The BAC libraries were each constructed from DNA derived from twenty five seedlings of an inbred cultivar Forrest.

	MTP4E	MTP4BH		MTP2 BH	Totals
Vector	pBeloBAC11	pCLD04541			na
Insertion site	Eco RI	BamHI or HindIII			na
Duplicates/region	1	1	2–4	1–4	na
Number of clones	3840	4608	576	8064	17 088
Mean insert size (kbp)	175 ± 7	173 ± 7	173 ± 7	140 ± 5	685
Clone coverage	0.7	0.8	0.2	1.4	3.1
BESs good reads	3 324	6772	924	13 473	25 123
BESs coverage (Mbp)	2.9	5.0	0.7	9.9	18.5