A Solanum lycopersicum × Solanum pimpinellifolium Linkage Map of Tomato Displaying Genomic Locations of R-Genes, RGAs, and Candidate Resistance/Defense-Response ESTs

Table 1

Listof ESTs mapped in the Solanum lycopersicum  ×  S. pimpinellifolium F₂ population, their putative function, chromosomal location, and copy number.

EST clone	^aSGN-TD	^bPutative function	Chr.	^cCopy no.
cTOF3A14	C146883	Cytosolic Cu, Zn Superoxide dismutase, S. lycopersicum	1	2
cTOE7J7a	C139397	Endo-1,4-beta-glucanase, S. lycopersicum	1	6
cLED27E12	C19568	Cold acclimation protein WCOR413-like protein form, O. sativa	1	2
cTOE6F10	C139034	Lipoxygenase, S. lycopersicum	1	5
cLEG9N2	C45935	Subunit A of ferredoxin-thioredoxin reductase, S. tuberosum	1	1
cLES9N20	C79709	ASC1 (Alternaria stem canker resistance protein), S. lycopersicum	1	1
cLEC6O2	C11013	Polyamine oxidase, A. thaliana	1	1
cTOF20P4	C142906	Carotenoid cleavage dioxygenase 1-2, S. lycopersicum	1	5
cLEZ11K12	C98684	Snakin2 precursor, S. lycopersicum	1	1
cTOA9E13	C117653	Squalene synthase, C. annuum	1	5
cTOA9C11	C117644	Similar to WRKY transcription factor Nt-SubD48, N. tabacum	2	1
cLET10E15	C79822	Acidic 26kDa endochitinase precursor, S. lycopersicum	2	1
cTOF19J9	C142319	Phosphoribosylanthranilate isomerase, A. thaliana	2	1
cLEY1K9	C97179	Pathogen-inducible alpha-dioxygenase, N. attenuata	2	4
cLEW11E20	C89000	Resistance complex protein I2C-3, S. lycopersicum	2	7
cTOF16A9	C141311	Calmodulin 3 protein, S. lycopersicum	3	9
cLER17H16	C71298	Elicitor-inducible cytochrome P450, N. tabacum	3	1
cTOF18P1	C142154	Serine palmitoyltransferase, S. tuberosum	3	3
cLEX12O16	C92852	Ethylene response factor 5, S. lycopersicum	3	6
cTOE2F15	C137984	Catalase isozyme 1, S. lycopersicum	3	1
cTOF29J22	C145412	4-coumarate-coA ligase 1, S. tuberosum	3	2
cLEX10F20	C92172	Ethylene response factor 1, S. lycopersicum	3	4
cTOF14B17	C141010	Anthocyanin 5-O-glucosyltransferase, S. sogarandinum	4	1
cLED15E5	C16128	Shikimate kinase chloroplast precursor, S. lycopersicum	4	1
cLEN13D5	C66215	Chorismate synthase 1 precursor, S. lycopersicum	4	4
cTOS21D12	C163577	Similar to heat shock factor, N. tabacum	4	3
cTOF10N11	C140057	Myo-inositol-1-phosphate synthase, S. lycopersicum	4	4/5
cLEW24M21	C90911	TMV disease resistance protein-like protein, Cicer arietinum	4	2
cLEW22D11b	C90352	4-coumarate:coenzyme A ligase, N. tabacum	4	10
cLER5E19	C73560	Phospholipase PLDb1, S. lycopersicum	5	1
cTOC2J14a	C127676	Disease resistance gene homolog Mi-copy1, S. lycopersicum	5	9
cTOC2J14b	C127676	Disease resistance gene homolog Mi-copy1, S. lycopersicum	5	9
cTOF26E9	C144413	Prf, S. pimpinellifolium	5	2
cTOE1K1	C136851	Spermidine synthase, S. lycopersicum	5	4
cTOE7J7b	C139397	Endo-1,4-beta-glucanase, S. lycopersicum	5	6
cTOF29B13	C145236	Metallothionein-like protein type 2 a, S. lycopersicum	5	2
cTOF33C3	C146601	Serine/threonine protein kinase Pto, S. lycopersicum	5	10
cTOF23J19	C143585	Heat shock protein 90, S. lycopersicum	5	4
cLEG32E10	C34795	Lipoxygenase B, S. lycopersicum	6	6
cTOF8F19	C148467	Ascorbate peroxidase, S. lycopersicum	6	2
cLEZ16H16	C99197	Contains similarity to disease resistance response protein, Pisum sativum	6	1
cLED11A2	C15134	Mitogen-activated protein (MAP) kinase 3, C. annuum	6	2
cLEW22D11a	C90352	4-coumarate:coenzyme A ligase, N. Tabacum	6	10
cLEY21L21	C97473	Disease resistance gene homolog Mi-copy1, S. lycopersicum	6	6
cLEW22N22	C90504	Ethylene-responsive element binding factor 6-N. sylvestris	6	3
cTOF34C13	C146804	Peroxiredoxin Q-like protein, A. thaliana	7	1
cLEN14F9	C66474	Sucrose-phosphate synthase, S. lycopersicum	7	1
cTOF21F12	C142982	Dehydroquinate dehydratase/shikimate, NADP oxidoreductase, S. lycopersicum	7	9
cLEN13G22	C66246	1-aminocyclopropane-1-carboxylate oxidase, S. lycopersicum	7	4
cLEY22L20	C97674	Peroxidase precursor, S. lycopersicum	7	3
cTOE15M9	C136013	MYB-related transcription factor VlMYBB1-1, Vitis labrusca  ×  V. vinifera	7	6
cLEG34O20	C35423	UDP-glucose:salicylic acid glucosyltransferase, N. tabacum	7	4
cLEN14C8	C66419	PR-related protein, PR P23 (salt-induced protein), S. lycopersicum	8	3
cTOF9D16	C148734	Pathogenesis-related protein 5-1, S. lycopersicum	8	1
cTOF28D12	C144993	Polyphenol oxidase E, chloroplast precursor, S. lycopersicum	8	7
cLEN10H3	C65539	Heat shock factor protein HSF8 (Heat shock transcription factor 8), S. lycopersicum	8	2
cLEI16E21	C47449	Cold-induced glucosyl transferase, S. lycopersicum	8	3
cTOF2N15	C145786	Osmotin-like protein OSML13 precursor (PA13), S. lycopersicum	8	3
cTOE23J12	C137767	Monodehydroascorbate reductase, S. lycopersicum	8	3
cLED27C20	C19537	DNADPH oxidase; gp91-phox homolog, S. lycopersicum	8	1
cLER14J12	C70373	WRKY transcription factor IId-1 splice variant 2, S. lycopersicum	8	1
cTOF2L16	C145747	Phenylalanine ammonia-lyase (PAL), S. lycopersicum	8	1
cTOD3N7	C132799	Endo-1,4-beta-glucanase, S. lycopersicum	8	2
cLEX11E19	C92435	Putative NADH-ubiquinone oxireductase, A. thaliana	9	1
cTOE10J18	C134749	PR protein sth-2, S. Tuberosum	9	3
cLEC13E21	C1592	P14 (PR-Protein), S. lycopersicum	9	3
cLEC6M14	C10964	PR-protein sth-2, S. Tuberosum	9	5
cLER14J6	C70387	Hexose transporter, S. lycopersicum	9	1
cTOF19O3	C142383	Hydroxyproline-rich glycoprotein homolog, A. thaliana	9	3
cLEZ6E21b	C100278	Ubiquitin, S. lycopersicum	10	4/5
cLED18G6	C17041	Similar to WRKY-like drought-induced protein, Retama raetam	10	6
cTOD4I20	C133021	Tyrosine aminotransferase, A. thaliana	10	2
cLHT11J12	C100975	Diacylglycerol kinase, S. lycopersicum	10	2
cLER4F5	C73337	Ferredoxin-I chloroplast precursor S. lycopersicum	10	4
cTOF30K21	C146034	Chloroplast ferredoxin I, S. lycopersicum	10	>10
cTOF22M16	C143336	NADH-ubiquinone oxidoreductase 23 kDa subunit, S. lycopersicum	10	1
cLEN14K6	C66563	Multiresistance protein homolog, A. thaliana	10	3/2
cLEX10N16	C92314	PR protein, S. lycopersicum	10	>10
cLEC18O1	C3034	Basic 30kDa endochitinase precursor, S. lycopersicum	10	>10
cTOF31H10	C146231	Catechol O-methyltransferase, N. tabacum	10	8
cLEN9P2	C69374	Multiresistance protein homolog, A. thaliana	10	2
cLED13I7	C15652	Resistance complex protein I2C-1, S. lycopersicum	11	7
cTOF28I23	C145097	Resistance complex protein I2C-5, S. pimpinellifolium	11	>10
cLEZ6E21a	C100278	Ubiquitin, S. lycopersicum	11	4/5
cTOF29F6b	C145330	10-hydroxygeraniol oxidoreductase, -S. lycopersicum	11	7
cLEC14I18a	C1998	Resistance complex protein I2C-2, S. lycopersicum	11	>10
cLEC14I18b	C1998	Resistance complex protein I2C-2, S. lycopersicum	11	>10
cLEM22K17	C62708	9-cis-epoxycarotenoid dioxygenase, S. lycopersicum	11	7
cLED23K21	C18512	Resistance complex protein I2C-5, S. lycopersicum	11	>10
cLES18N16	C76694	Phosphatidylinositol 4-kinase, S. tuberosum	11	2
cTOS21D14	C163579	WRKY transcription factor IId-2, S. lycopersicum	12	1
cLPT1G11	C109877	S-adenosyl-l-homocysteine hydrolase, S. lycopersicum	12	4
cLEZ15E8	C98979	Extensin class I, S. Lycopersicum	12	>10
cLEW25D9	C90989	Glutamine synthetase, S. lycopersicum	12	3

^aSolanaceae Genome Network (SGN) can be accessed at http://www.sgn.cornell.edu/.
^bThe putative function of each EST has been derived from Computational Biology and Functional Genomics Laboratory web site (http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/est_report.pl), used to be maintained at The Institute for Genomic Research (TIGR). (Computational Biology and Functional Genomics Laboratory).
^cThe exact or approximate copy number of ESTs in tomato genome was determined based on the number of hybridized bands on Southern blot gels and may be varied in different labs. Where there is a “/” sign, the figures in the left side denote the number of copies in S. lycopersicum parent and those in the right side denote the number of copies in S. pimpinellifolium parent.