Reproducible RNA Preparation from Sugarcane and Citrus for Functional Genomic Applications
Figure 4
Chip-based electrophoretic separation of amplified total RNA (aRNA) isolated by the TENS-PCI method. aRNA was analyzed using an Agilent 2100 Bioanalyzer. A total of 20 ng of aRNA was loaded per well. (a) Densitometry simulation for aRNA from stem, leaf and root tissues of untreated plants as well as from stem tissues of plants after treatment with SA (5 mM) or MeJA (100 M) at 0, 24 and 48 hours (h). (b) Representative profiles of aRNA corresponding to stems of untreated plants, as well as to stems of control (mock: water and 0.1 % ethanol, 48 hours) and treated plants (MeJA at 48 hours). The Agilent RNA 6000 Nano ladder is included for sizing.
