Reproducible RNA Preparation from Sugarcane and Citrus for Functional Genomic Applications

Table 1

The TENS-PCI protocol used for RNA isolation.

(1) Extraction	(a) TENS(a) and PCI(b) (equal volume)
	(b) PCI
(2) Precipitation	3 M sodium acetate (pH 5.2) (0.1x volume)
	Ethanol (3.0x volume)
(3) Re-precipitation	4 M lithium chloride

(a)TENS: 10 mM hydroxymethyl aminomethane (Tris-HCl) (pH 7.5), 1 mM ethylenedinitrilo-tetraacetic acid (sodium EDTA) (pH 8.0), 0.1 M sodium chloride (NaCl), 1% (w/v) sodium dodecyl sulfate (SDS), 2% (w/v) polyvinylpyrrolidone (PVP)-40, and 7% (v/v) 2-mercaptoethanol.
(b)PCI: phenol (pH 4.3): chloroform: isoamyl alcohol (1.0: 0.8: 0.2).