Heterologous Expression of a Membrane-Spanning Auxin Importer: Implications for Functional Analyses of Auxin Transporters
Figure 5
Expression of epitope-tagged AUX1 in P. pastoris. P. pastoris strain KM71H transformed with plasmids encoding L2-His63xFLAG-AUX1 or N-His63xFLAG-AUX1 was grown, induced, and harvested as described in the Methods. Of the 32 cell lysates analysed by Western blotting (anti-FLAG antibody), only 6 are shown here for clarity, the other 26 showing no AUX1 expression. AUX1 was observed in a single N-His63xFLAG-AUX1 culture (lane 5). A number of nonspecific bands reacting with the anti-FLAG antibody are denoted with asterisks, including one migrating just higher than the AUX1 band.
