Methodologies for In Vitro Cloning of Small RNAs and Application for Plant Genome(s)

<table>Linear regression of total RNA quality (RIN) and the relative mass of the small RNA population determined for forty human, mouse and rat tissues. A significant negative correlation coefficient, <svg height="11.125" id="M3" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 76.387497 11.125" width="76.387497" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>, df = 38, derived from the regression indicates that total RNA quality is an essential component of small RNA cloning in that higher quality RNA retains a more pure small RNA fraction but, as a corollary, that enrichment of the small RNA fraction prior to cloning is crucial to success.</table>

International Journal of Plant Genomics

fig1

Figure 1

Figure 1: Methodologies for In Vitro Cloning of Small RNAs and Application for Plant Genome(s) 