Proteomic Analysis of Soybean Roots under Aluminum Stress

Table 1

Aluminum regulated-proteins detected in soybean line PI 416937 in time-course Al stress experiment.

Spot	Accession	Protein ID/functional class^a	Average expression fold change^b
			6 h	SE	51 h	SE	72 h	SE
5	gi/126411	Lipoxygenase (PM)	−1.04	0	−1.43	0.09	−1.25	0.07
8	gi/42820320	Copper amino oxidase (DD)	−1.04	1.05	−1.53	0.02	−2.24	0.08
15	gi/225425555	Thioredoxin (DD)	1.11	0.02	3.01	0.07	4.34	1.04
16	gi/224115920	Protein phosphatase 2A (ST)	1.13	0	1.83	0.14	3.05	0.4
17	gi/22331670	Serine/threonine protein kinase (ST)	−1.24	0.12	1.14	0.06	1.47	0.05
18	gi/15225438	Malate oxidoreductase (E)	−1.13	0.07	1.10	0.04	1.33	0.04
20	gi/162458207	Enolase (E)	1.07	0.04	1.04	1.12	1.39	0.03
21	gi/223548395	26S protease subunit 6a (PD)	−1.13	0.01	−1.29	0.04	−1.31	0.19
22	gi/2511541	DNA-binding protein gbp16 (UN)	1.03	—	1.48	0.21	1.90	0.23
23	gi/4836923	CREB binding protein (TF)	−1.05	0.03	−1.08	1.21	1.38	0.14
24	gi/223548531	S-adenosyl methionine synthase (PM)	1.13	1.17	1.41	0.35	1.45	0.14
25	gi/224285989	Amidase/tetratricopeptide repeat (PM)	−1.08	0.14	1.62	0.07	nd	—
26	gi/3024127	S-adenosyl methionine synthase (PM)	1.11	0.06	1.35	0.23	1.50	0.26
27	gi/223548639	Transaldolase (PM)	1.07	0.02	1.16	0.01	1.43	0.14
30	gi/211970690	Formate dehydrogenase (PM)	1.14	0.01	1.45	0.17	1.22	0.21
31	gi/1498340	Actin (CS)	1.06	0.02	1.35	0	1.05	1.37
33	gi/157346459	NADH:flavin oxidoreductase (DD)	1.05	1.08	1.49	0.09	1.30	0.1
34	gi/242462	Lipoxygenase (PM)	nd	—	1.71	0.08	1.62	0.3
35	gi/195637880	Pyruvate dehydrogenase E1 (E)	1.02	1.03	1.30	0.04	1.30	0.01
36	gi/9230771	Cyt phosphoglysrate kinase (PM)	nd	—	1.59	0.21	1.27	0.11
37	gi/49257111	Disulfide isomerase-like (PD)	−1.12	1.26	−1.63	0.02	1.02	1.32
38	gi/168029670	Unknown (UN)	nd	—	nd	—	1.61	0.38
39	gi/126508778	Cystine synthase (PM)	1.08	0.04	1.40	0.16	1.46	0.16
42	gi/3273828	Malate dehydrogenase (E)	1.03	1.1	1.87	0.55	2.05	0.62
43	gi/186506243	Aaldo/keto reductase (PM)	1.14	0.06	1.52	0.1	1.48	0.27
44	gi/34559418	(+)-pulegone reductase (SM)	1.09	1.15	1.31	0.24	1.43	0.26
45	gi/108864466	Unknown (UN)	nd	—	1.64	0	1.85	0.09
46	gi/2687724	NADPH:isoflavone reductase (SM)	1.24	0.22	nd	—	2.17	0.33
50	gi/393401	Alpha tubulin (CS)	1.09	0.07	−1.45	0.05	−1.30	0.09
55	gi/3023196	14-3-like protein C (ST)	nd	—	nd	—	1.41	0.27
56	gi/6469121	Plasma membrane polypeptide (UN)	nd	—	nd	—	1.30	0.07
61	gi/186507172	BRCA1 C terminal (CD)	1.09	0.04	1.21	0.04	1.41	0.01
62	gi/6690745	Resistance protein (DD)	nd	—	nd	—	3.81	0.04
64	gi/3023194	14-3-3-like protein A (ST)	1.11	0.08	−1.15	0.06	1.60	2.11
65	gi/125550993	Unknown (UN)	1.00	1.01	−1.48	0.02	−1.28	0
67	gi/225455804	Hypothetical protein (UN)	1.04	1.09	1.61	0.12	1.22	0.03
70	gi/226866	31 kD protein (UN)	2.19	0.67	−1.28	1.81	−1.31	4.14
75	gi/11385431	Glutathione S-transferase 8 (DD)	−1.04	1.06	1.39	0.07	1.16	1.17
78	gi/224087343	WD40 protein/RNA processing (TF)	−1.06	1.15	1.34	0.15	1.18	0.02
79	gi/194705252	Cytidyly transferase (SM)	−1.25	0.08	1.64	0.24	1.00	0.9
81	gi/1170781	Glutathatione-S-transferase 3 (DD)	1.10	1.64	4.05	0.92	2.27	2.03
82	gi/11385435	Glutathione-S-transferase 10 (DD)	1.12	1.23	2.30	0.16	1.63	0.75
83	gi/399240	20 kDa chaperonin (UN)	−1.02	1.39	2.29	0.33	1.54	2.14
84	gi/224135489	Beta glucosidase (PM)	1.16	0.03	nd	—	4.02	0.48
85	gi/11385459	Gluthstione-S-transferase 22 (DD)	−1.07	1.16	1.55	0.01	1.59	0.26
89	gi/28192427	Dehydroascorbate reductase (DD)	1.09	0.049	1.35	0.05	−1.08	1.62
90	gi/30682123	Adenine phosphoribosyl transferase, ATP4 (PM)	1.24	0.01	1.38	0.02	nd	—
91	gi/17380185	Proteasome subunit beta-1 (PD)	−1.07	1.16	1.42	0.14	nd	—
92	gi/21068664	Quinine oxidoreductase (DD)	1.21	0.01	−1.20	0.14	−1.31	0.14

^aPM: primary metabolism, SM: secondary metabolism, E: energy, PD: protein destination, TF: transcription factor, CS: cell structure, ST: signal transduction, DD: disease/defense, CD: cell division, UN: unknown function;
^bexpression fold change at each time point, negative values: downregulation, positive values: upregulation, fold change significance cut-off is ±1.3, SE: standard error, nd: not detected.