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International Journal of Plant Genomics
Volume 2012 (2012), Article ID 417935, 12 pages
http://dx.doi.org/10.1155/2012/417935
Research Article

Evolutionary and Molecular Aspects of Indian Tomato Leaf Curl Virus Coat Protein

1Department of Bioinformatics, Applied Botany Center, University School of Sciences, Gujarat University, Ahmedabad 380 009, India
2Department of Botany, University School of Sciences, Gujarat University, Ahmedabad 380 009, India

Received 24 August 2012; Revised 7 November 2012; Accepted 8 November 2012

Academic Editor: Akhilesh Kumar Tyagi

Copyright © 2012 Sivakumar Prasanth Kumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tomato leaf curl disease (ToLCD) is manifested by yellowing of leaf lamina with upward leaf curl, leaf distortion, shrinking of the leaf surface, and stunted plant growth caused by tomato leaf curl virus (ToLCV). In the present study, using computational methods we explored the evolutionary and molecular prospects of viral coat protein derived from an isolate of Vadodara district, Gujarat (ToLCGV-[Vad]), India. We found that the amino acids in coat protein required for systemic infection, viral particle formation, and insect transmission to host cells were conserved amongst Indian strains. Phylogenetic studies on Indian ToLCV coat proteins showed evolutionary compatibility with other viral taxa. Modeling of coat protein revealed a topology similar to characteristic Geminate viral particle consisting of antiparallel β-barrel motif with N-terminus α-helix. The molecular interaction of coat protein with the viral DNA required for encapsidation and nuclear shuttling was investigated through sequence- and structure-based approaches. We further emphasized the role of loops in coat protein structure as molecular recognition interface.