Poor Homologous Synapsis 1 Interacts with Chromatin but Does Not Colocalise with ASYnapsis 1 during Early Meiosis in Bread Wheat

Figure 4

Q-PCR profiling of TaPHS1 shows that it is expressed during meiosis. While the amount of TaPHS1 mRNA transcript is low, it has higher levels of expression during premeiosis when compared to the other stages of meiosis examined in Chinese Spring wild type (open bars). In the ph1b mutant (black bars), TaPHS1 is upregulated between 1.5- and 2-fold across the time points analysed. Normalisation of the Q-PCR data was performed against three control genes (actin, GAPdH, and cyclophilin) as per Crismani et al. [36]. Data represent the means ± SE of three replicates. Units on the y-axis represent normalised mRNA transcript μL⁻¹.