A Cotton-Fiber-Associated Cyclin-Dependent Kinase A Gene: Characterization and Chromosomal Location
Figure 3
(a) Northern analysis of GhCDKA expression in different cotton tissues. Ten μg of total RNA from fibers (5, 10, 15, and 20 DPA, lanes 1–4), flowers (lane 5), leaves (lane 6), and roots (lane 7) was electrophoresed on an agarose gel, transferred onto a nylon membrane, and hybridized with a 32P-labeled GhCDKA cDNA. Two EtBr-stained rRNA bands indicate that an equal amount of total RNA was loaded for each sample. The relative GhCDKA transcript levels were determined by the ratio of hybridized intensity of the 1.2 kb GhCDKA mRNA to the EtBr stained 28S rRNA band using the program of Scion Image for Windows (Scion Corporation). (b) RT-PCR analysis of GhCDKA mRNA. Total RNA from leaves, flowers, roots, and 5, 10, and 15 DPA fibers was used as template in generating first strand cDNA. Each cDNA was made 1x, 5x, 10x, and 20x dilutions and used as template for PCR amplification with two GhCDKA gene specific primers: CDKC-2 and CDK5-1.
