Phylogenetic, Molecular, and Biochemical Characterization of Caffeic Acid o-Methyltransferase Gene Family in Brachypodium distachyon
Figure 5
SDS-PAGE gel analysis of purification of recombinant BdCOMTs in E. coli. (a) Expression and purification of BdCOMT4 in E. coli. Lane M, protein size standards. Lane 1, total lysate of BL21(DE2) pLysS E.coli. containing pQE-BdCOMT1 before IPTG induction. Lane 2, total lysate of BL21(DE3)pLysS E. coli. containing pQE-BdCOMT1 with 1 mM IPTG induction for 4 hrs. Lane 3, flow-through lysate. Lanes 4–8, BdCOMT1 protein samples collected in different 100 L fractions after adding the elution buffer. (b) SDS-PAGE gel analysis of purified BdCOMT proteins. Lane M, Protein size standards. Lane 1, 3 μg BdCOMT4; Lane 2, 3 μg BdCOMT2; Lane 3, 3 μg BdCOMT3; Lane 4, 3 μg BdCOMT1.
