Application of Microsatellite Loci for Molecular Identification of Elite Genotypes, Analysis of Clonality, and Genetic Diversity in Aspen Populus tremula L. (Salicaceae)

Figure 2

(a) Electrophoretic patterns of PCR-amplified SSR loci ORPM202, ORPM206, ORPM220, ORPM296, and ORPM312 in aspen. Loci: lanes 1–4: ORPM202; lanes 6–9: ORPM206; lanes 10–13: ORPM220; samples: 1, 6, 10, 15, and 19, aspen from native population; 2, 7, 11, 16, and 20, clone С-control; 3, 8, 12, 18, and 21, clone 47-1; 4, 9, 13, 18, and 22, clone PtV22. Lanes 5, 14, and 23: DNA molecular weight marker (DNA of E. coli plasmid pBR322, digested by restriction endonuclease HpaII). (b) Electrophoretic patterns of PCR-amplified SSR loci WPMS15, WPMS17, WPMS18, WPMS19, WPMS21, and WPMS22 in aspen. Loci: lanes 1–4: WPMS15, lanes 6–9: WPMS17, lanes 10–13: WPMS18, lanes 15–18: WPMS19, lanes 19–22: WPMS21, and lanes 24–27: WPMS22. Samples: 1, 6, 10, 15, 19, and 24, aspen from natural population; 2, 7, 11, 16, 20, and 25, clone С-control; 3, 8, 12, 16, 21, and 26, clone 47-1; 4, 9, 13, 18, 22, and 27, clone PtV22. Lanes 5, 14, and 23, DNA molecular weight marker (DNA of E. coli plasmid pBR322, digested by restriction endonuclease HpaII).

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