Application of Microsatellite Loci for Molecular Identification of Elite Genotypes, Analysis of Clonality, and Genetic Diversity in Aspen Populus tremula L. (Salicaceae)

Table 2

Microsatellite loci tested for PCR amplification in aspen.

Loci	Primer sequences (5′- 3′)	Repeat motif	Fragment size (bp)^3
ORPM14^1	F- GGGCTGCAGCAGATATTGA; R- CCAAAGGAACCCAAAGAAGA	(GCTC)4	146–162
ORPM18^1	F- AGCAGAGATCGATGCTGAGG; R- AATTTCTCGCTTCTCGCATT	(TTTA)4	205
ORPM36^1	F- AGCCTCCAAACACCATGAAC; R- ACAGTGGTGTGGATCCTGCT	(GAAA)4	213
ORPM60^1	F- ATAGCGCCAGAAGCAAAAAC; R- AAGCAGAAAGTCGTAGGTTCG	(AAT)5	212
ORPM79^1	F- GAAGCTGAAAACAACAACAAACA; R- GGGTTTTTAACATAATAAAAGCTTGG	(AAT)4	160
ORPM81^1	F- GCTGCAGCCAAACAAAGC; R- CAGAAATCCCACCCAAACC	(TATT)4	142–158
ORPM84^1	F- CTGCAGCCTTACCACCATTT; R- CGTGTTCGAGATTGGGATTT	(AAG)4	171
ORPM86^1	F- CCACATCCATAGCTCTGCAAC; R- GTACTACCTCGCCTGCCAAC	(CTT)5	204
ORPM107^1	F- AATCTGGTGGCTTGCCTCT; R- TTGAGGAACACGTGCAGACT	(TAAA)4	190
ORPM117^1	F- CCCCCTAATTACCTTGGAAAC; R- TTGTTTGTATCTCCTCCGTTGA	(ATTA)4	210
ORPM158^1	F- GCTGAAACATCCTTCATGGTC; R- CGAAGCTGCATAAGCATCAA	(TTTC)4	200
ORPM193^1	F- CCGCTGGATTTGTTTGTTTT; R- TGAGCAGAAAGATGCGAAGA	(ATTTT)4	187–207
ORPM202^1	F- TCGCAAAAGATTCTCCCAGT; R- TTCAAATCCCGGTAATGCTC	(TAA)5	184–190
ORPM206^1	F- CCGTGGCCATTGACTCTTTA; R- GAACCCATTTGGTGCAAGAT	(GCT)7	190–208
ORPM220^1	F- AGCTAGCCTGTCGTCAAGGA; R- CAAGGAAGCATTCTCGCAAT	(TTTA)6	178–222
ORPM296^1	F- CGAAGCCATTGACCCAGTAT; R- GGGCATCTCTCTCCTTTCAA	(GTTCTG)4	199
ORPM312^1	F- GTGGGGATCAATCCAAAAGA; R- CCCATATCAAACCATTTGAAAAA	(CCT)6	194
ORPM366^1	F- CCTTGAGGGGACACTTCGAT; R- AAAGAGTTGAGCCCTTGGTG	(TTA)5	156
ORPM371^1	F- CCGGACTCTCACAAATCTCC; R- TGCTTTTGCTCCTGGTTCTT	(TCTT)6	192–200
ORPM372^1	F- AGCTCTTCTGCTGGTGCTGT; R- GAGGGAGGGAGGGTAAAAGA	(TCTT)5	190
ORPM415^1	F- CTCGGTGCAAATATCGGTTC; R- AGATCGATGGTCCTTTCCTG	(GGCG)4	225
ORPM484^1	F- CAAAATGGCAATCCAAGGTT; R- CCAAGCTTCCAATTGAGTCC	(TTAA)4	190–206
ORPM488^1	F- CTCCAGCCGCTTCTATCCTT; R- TGTCGTGGGAAAGAACCAGT	(TTA)6	200
ORPM496^1	F- CAGCAGTGCAAGCTCCTAAA; R- GGCCACTGACAGAGACCAAG	(GGA)4	185
WPMS14^2	F- CAGCCGCAGCCACTGAGAAATC; R- GCCTGCTGAGAAGACTGCCTTGAC	(CGT)28	215–287
WPMS15^2	F- CAACAAACCATCAATGAAGAAGAC; R- AGAGGGTGTTGGGGGTGACTA	(CCT)14	201–219
WPMS16^2	F- CTCGTACTATTTCCGATGATGACC; R- AGATTATTAGGTGGGCCAAGGACT	(GTC)8	139–184
WPMS17^2	F- ACATCCGCCAATGCTTCGGTGTTT; R- GTGACGGTGGTGGCGGATTTTCTT	(CAC)15	122–146
WPMS18^2	F- CTTCACATAGGACATAGCAGCATC; R- CACCAGAGTCATCACCAGTTATTG	(GTG)13	219–248
WPMS19^2	F- AGCCACAGCAAATTCAGATGATGC; R- CCTGCTGAGAAGACTGCCTTGACA	(CAG)28	180–234
WPMS20^2	F- GTGCGCACATCTATGACTATCG; R- ATCTTGTAATTCTCCGGGCATCT	(TTCTGG)8	210–222
WPMS21^2	F- TGCTGATGCAAAAGATTTAG; R- TTGGAACTTCAACATTCAGAT	(GCT)45	287–326
WPMS22^2	F- ACATGCTACGTGTTTGGAATG; R- ATCGTATGGATGTAATTGTCTTA	(TGA)23	100–135

Comments: ¹Tuskan et al., 2004 [15]; ²Smulders et al., 2001 [16]; ³by literature data in different Populus species (P. tremula, P. x canescens, and P. alba).