Identification and DUS Testing of Rice Varieties through Microsatellite Markers

Table 2

Characteristics of polymorphic microsatellite markers used in this study, including locus name, number of chromosome, primer sequences, number of alleles, effective number of alleles, polymorphic information content (PIC), and discriminating power ().

Number	Locus name	Chromosome number	Primer sequences	Number of alleles	Effective number of alleles	PIC
1	RM11	1	F: CAAATCCCGACTGCTGTCC; R: TGGGAAGAGAGCACTACAGC	7	4.15	0.73	0.78
2	RM44	2	F: ACCCTCTCCGCCTCGCCTCCTC; R: CTCCTCCTCCTGCGACCGCTCC	8	5.55	0.8	0.8
3	RM55	3	F: CCGTCGCCGTAGTAGAGAAG; R: TCCCGGTTATTTTAAGGCG	3	2.73	0.6	0.68
4	RM124	4	F: ATCGTCTGCGTTGCGGCTGCTG; R: CATGGATCACCGAGCTCCCCCC	8	5.81	0.8	0.83
5	RM133	5	F: TGCAGATGAGAAGCGGCGCCTC; R: TGTGTCATCAGACGGCGCTCCG	4	3.16	0.63	0.73
6	RM154	6	F: TTGGATTGTTTTGCTGGCTCGC; R: GGAACACGGGGTCGGAAGCGAC	6	5.32	0.79	0.82
7	RM161	7	F: TCTCCTCTTCCCCCGATC; R: ATAGCGGGCGAGGCTTAG	4	2.44	0.52	0.57
8	RM237	8	F: ACGGGCAATCCGAACAACC; R: TCGGGAAAACCTACCCTACC	5	4.63	0.75	0.8
9	RM271	9	F: CTAGTTGGGCATACGATGGC; R: ACGCTTATATGTTACGTCAAC	10	6.58	0.83	0.88
10	RM277	10	F: TCAGATCTACAATTCCATCC; R: TCGGTGAGACCTAGAGAGCC	7	4.6	0.76	0.8
11	RM287	11	F: TTCCCTGTTAAGAGAGAAATC; R: GTGTATTTGGTGAAAGCAAC	8	4.4	0.74	0.78
12	RM316	12	F: CGGTCAAATCATCACCTGAC; R: CAAGGCTTGCAAGGGAAG	13	9.52	0.9	0.93
Average				6.91	4.90	0.73	0.78