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International Journal of Proteomics
Volume 2010 (2010), Article ID 726968, 14 pages
http://dx.doi.org/10.1155/2010/726968
Research Article

Comparative Proteomic Analysis of Proteins Involved in the Tumorigenic Process of Seminal Vesicle Carcinoma in Transgenic Mice

1Genomics Research Center, Academia Sinica, Taipei, Taiwan
2National Applied Research Laboratories, National Laboratory Animal Center, Taipei, Taiwan
3Institute of Information Science, Academia Sinica, Taipei, Taiwan
4Department of Medical Research, Mackay Memorial Hospital, Tamshui, Taiwan
5Chemistry Department, National Taiwan University, Taipei, Taiwan
6Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, Taiwan

Received 18 December 2009; Accepted 18 February 2010

Academic Editor: Alexander I. Archakov

Copyright © 2010 Wei-Chao Chang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We studied the seminal vesicle secretion (SVS) of transgenic mice by using one-dimensional gel electrophoresis combined with LTQ-FT ICR MS analysis to explore protein expression profiles. Using unique peptide numbers as a cut-off criterion, 79 proteins were identified with high confidence in the SVS proteome. Label-free quantitative analysis was performed by using the IDEAL_Q software program. Furthermore, western blot assays were performed to validate the expression of seminal vesicle proteins. Sulfhydryl oxidase 1, glia-derived nexin, SVS1, SVS3, and SVS6 showed overexpression in SVS during cancer development. With high sequence similarity to human semenogelin, SVS2 is the most abundance protein in SVS and is dramatically decreased during the tumorigenic process. Our results indicate that these protein candidates could serve as potential targets for monitoring seminal vesicle carcinoma. Moreover, this information can provide clues for investigating seminal vesicle secretion-containing seminal plasma for related human diseases.