International Journal of Proteomics

Research Article

Comparative Proteomic Analysis of Proteins Involved in the Tumorigenic Process of Seminal Vesicle Carcinoma in Transgenic Mice

Table 1

Label-free quantitative analysis of mouse SVS proteome.


Accession number			Unique Peptide	(A) protein aboudance				(B) average CV of peptides (%)				(C) relative expression ratio			(D) percentage of content				(E) Western blot assay
	Protein	Mass/pI	Unique Peptide	Hp	Ad	Ac	N	Hp	Ad	Ac	N	Hp/N	Ad/N	Ac/N	Hp	Ad	Ac	N

Seminal vesicle protein

IPI00408931	Carcinoembryonic antigen-related cell adhesion molecule 10	29455/6.84	24					12.4	4.3	4.2	10.0	0.57	0.50	0.63	2.50	2.06	2.72	1.30	●
IPI00115065	Glia-derived nexin	44179/9.85	44					11.7	7.7	12.5	17.6	1.76	2.18	2.00	0.42	0.49	0.47	0.07	●
IPI00469211	Sulfhydryl oxidase 1, isoform 3	63296/8.17	53					7.2	3.6	3.8	14.3	1.00	1.45	1.74	0.51	0.69	0.88	0.15	●
IPI00347009	Secreted seminal- vesicle Ly-6 protein 1	11147/8.72	7					31.1	6.3	11.5	22.6	1.10	1.61	0.93	0.15	0.21	0.13	0.04	●
IPI00133448	Seminal vesicle antigen	18266/5.57	26					12.0	8.1	5.6	19.7	0.40	0.60	0.29	2.97	4.08	2.13	2.17
IPI00227548	Seminal vesicle secretory protein 1	93470/8.98	62					9.1	4.2	5.7	15.8	3.02	3.41	4.42	0.98	1.03	1.43	0.10	●
IPI00331164	Seminal vesicle secretory protein 2	40824/9.89	203					6.7	3.6	4.3	10.9	0.12	0.12	0.09	32.95	31.06	26.67	83.74	●
IPI00227543	Seminal vesicle secretory protein 3 beta	29983/9.37	42					9.6	7.9	3.9	13.1	2.03	2.24	2.71	3.41	3.51	4.51	0.49	●
IPI00314341	Seminal vesicle secretory protein 3a	29948/9.39	7					28.7	18.1	13.0	12.9	1.60	2.21	3.03	0.12	0.15	0.22	0.02	●
IPI00134286	Seminal vesicle secretory protein 4	12507/8.04	18					7.4	7.4	3.9	12.5	1.08	0.93	1.34	1.43	1.15	1.76	0.39
IPI00135709	Seminal vesicle secretory protein 5	13011/9.52	22					8.7	8.5	4.8	14.2	0.68	0.60	0.36	1.23	1.01	0.65	0.53	●
IPI00133431	Seminal vesicle secretory protein 6	11475/5.93	15					13.4	8.7	5.8	12.0	1.38	1.35	1.05	0.20	0.18	0.15	0.04
IPI00877305	Seminal vesicle secretory protein 7	11060/8.93	21					6.2	9.5	6.9	14.2	0.70	0.70	0.45	1.06	0.99	0.67	0.45	●
IPI00229083	Serine protease inhibitor kazal-like protein	10535/7.55	6					13.2	11.9	12.7	14.5	0.81	0.81	0.51	0.14	0.13	0.09	0.05	●
IPI00132416	Serine protease inhibitor Kazal-type 3	8482/8.26	7					4.5	6.3	7.0	7.7	0.59	0.73	0.48	0.57	0.67	0.46	0.29	●
IPI00308892	similar to lysozyme C, type M	17039/9.70	17					17.3	32.9	21.1	12.1	0.78	0.25	0.13	0.43	0.13	0.07	0.16	●

Serum protein and Secreted protein

IPI00131695	Albumin	68648/5.75	109					12.4	4.6	4.6	12.1	3.74	2.86	1.62	8.34	5.94	3.57	0.66	●
IPI00129755	Alpha-1-antitrypsin 1-2	45946/5.32	8					6.6	6.3	5.5	27.5	0.58	0.57	0.24	0.08	0.07	0.03	0.04
IPI00123924	Alpha-1-antitrypsin 1-4	45969/5.24	24					14.9	6.7	7.1	16.4	1.20	1.54	0.91	0.87	1.05	0.65	0.21
IPI00123927	Alpha-1-antitrypsin 1-5	45862/5.44	9					25.5	4.7	27.1	10.6	0.27	0.71	0.72	0.08	0.20	0.22	0.09
IPI00117857	Alpha-1-antitrypsin 1-6	45794/5.25	10					7.9	7.5	5.8	43.6	4.32	3.51	1.67	0.15	0.11	0.06	0.01
IPI00128249	Alpha-2-HS-glycoprotein	37302/6.04	9					6.0	16.7	9.0	13.9	0.61	0.66	0.63	0.08	0.08	0.08	0.04
IPI00624663	Alpha-2-macroglobulin	167177/6.38	11					15.2	7.1	6.2	15.8	1.01	1.27	1.53	3.79	4.43	5.69	1.11
IPI00169625	Antiacid phosphatase variable light chain 18	16955/8.50	5					ND	ND	ND	0.0	ND	ND	ND	ND	ND	ND	0.00
IPI00136642	Antithrombin-III	51971/6.10	13					32.0	9.2	3.0	12.3	1.26	2.10	4.35	0.03	0.05	0.12	0.01
IPI00877236	Apolipoprotein A-I	30569/5.64	37					15.4	10.2	9.5	20.4	3.80	5.04	9.13	0.41	0.51	0.97	0.03
IPI00377351	Apolipoprotein A-IV	45001/5.41	23					16.7	17.6	19.8	20.7	1.10	1.09	1.36	2.94	2.69	3.59	0.78
IPI00322463	Beta-2-glycoprotein 1	38593/8.59	9					29.5	5.6	8.2	36.4	14.67	7.08	9.01	0.06	0.03	0.04	0.00
IPI00331286	Beta-2-microglobulin	13770/8.55	15					7.5	6.4	6.0	10.0	0.88	1.21	0.92	0.88	1.13	0.92	0.30
IPI00320420	Clusterin	51623/5.46	47					23.8	11.0	10.3	28.0	4.38	2.45	4.81	1.94	1.01	2.11	0.13
IPI00323624	Complement C3, isoform Long (Fragment)	186538/6.29	53					18.4	9.1	9.3	25.8	1.49	1.70	1.75	1.45	1.55	1.69	0.29
IPI00130010	Complement component factor H	141179/6.85	22					14.8	19.1	5.0	15.1	1.26	1.54	0.53	1.20	1.37	0.50	0.28
IPI00114065	Complement factor B (Fragment)	84951/7.18	9					14.4	6.4	4.8	14.2	2.25	2.27	2.60	0.16	0.15	0.18	0.02
IPI00123744	Cystatin-C	15521/9.18	10					8.7	9.9	6.1	33.9	2.26	2.41	1.94	0.11	0.11	0.09	0.01
IPI00331487	Dickkopf-like protein 1	26623/7.98	6					14.3	8.1	23.7	8.7	8.46	8.77	4.16	0.03	0.03	0.01	0.00
IPI00115522	Fibrinogen, alpha	61288/7.16	13					7.2	12.9	9.2	12.3	1.06	1.27	2.71	0.28	0.31	0.70	0.08
IPI00279079	Fibrinogen, beta	54718/6.68	9					22.6	18.5	22.8	13.0	22.09	18.40	15.67	0.90	0.70	0.63	0.01
IPI00122312	Fibrinogen, gamma	50317/5.38	16					14.2	5.0	9.4	6.9	2.67	2.28	1.60	0.07	0.05	0.04	0.01
IPI00113539	Fibronectin	272319/5.39	11					8.0	1.1	12.7	10.6	0.16	0.29	0.20	0.05	0.08	0.06	0.09
IPI00117167	Gelsolin, isoform 1	85888/5.83	5					33.6	22.8	22.4	17.9	3.84	3.05	3.39	0.16	0.12	0.14	0.01
IPI00124640	Granulin	64972/6.70	17					10.5	3.6	15.6	18.6	1.70	1.92	2.25	0.20	0.21	0.26	0.03
IPI00409148	Haptoglobin	38727/5.88	7					2.8	8.7	10.7	13.5	1.32	1.54	1.63	0.03	0.04	0.04	0.01
IPI00469114	Hemoglobin, alpha	15076/7.96	7					7.4	6.9	6.1	24.0	2.15	2.79	6.49	3.07	3.70	9.15	0.42
IPI00110658	Hemoglobin, beta	15193/8.96	5					2.8	15.0	7.9	32.4	1.92	4.51	6.60	2.00	4.36	6.78	0.31
IPI00128484	Hemopexin	51308/7.92	27					19.5	11.5	3.0	7.8	2.87	11.10	12.23	0.22	0.80	0.94	0.02
IPI00322304	Histidine-rich glycoprotein HRG	60401/7.26	11					6.4	4.4	5.2	16.7	0.34	0.77	0.37	1.54	3.28	1.67	1.34
IPI00114958	Isoform HMW of Kininogen-1	73056/6.05	11					14.1	16.4	9.7	19.8	2.79	3.25	1.83	2.63	2.84	1.70	0.28
IPI00317340	Lactotransferrin	77788/8.86	35					26.4	21.3	18.6	18.6	1.46	1.14	0.84	2.70	1.97	1.54	0.55
IPI00114403	Metalloproteinase inhibitor 1	22613/9.14	5					13.4	4.4	6.6	26.0	0.72	0.69	0.79	0.24	0.22	0.26	0.10
IPI00123223	Murinoglobulin-1	165193/6.00	19					5.2	10.5	3.9	18.9	0.92	1.43	1.57	0.44	0.64	0.74	0.14
IPI00115941	Neutrophil gelatinase-associated lipocalin	22861/8.96	16					4.8	5.0	4.8	14.6	1.65	3.03	2.72	0.23	0.39	0.37	0.04
IPI00309704	Nucleobindin-2	50273/5.05	24					9.6	14.1	6.8	16.2	1.34	5.78	2.83	0.07	0.27	0.14	0.01
IPI00322936	Plasminogen	90723/6.21	16					6.2	4.5	4.3	14.3	1.39	1.50	1.13	0.03	0.03	0.02	0.01
IPI00135156	Protein FAM3B	26135/8.97	11					19.9	14.0	7.0	13.8	2.04	1.46	1.62	0.08	0.05	0.06	0.01
IPI00311104	Semaphorin-3C	85206/8.86	5					41.7	27.1	32.4	50.4	4.82	2.63	4.91	0.03	0.02	0.03	0.00
IPI00131830	Serine protease inhibitor A3K	46850/5.05	19					13.5	8.2	4.7	18.0	16.28	16.63	7.69	0.11	0.11	0.05	0.00
IPI00139788	Serotransferrin	76674/6.94	90					13.6	7.6	13.7	16.3	2.99	2.62	2.20	1.50	1.22	1.09	0.15
IPI00309214	Serum amyloid P-component	26230/5.98	5					47.5	36.3	24.6	6.4	1.17	1.08	0.65	0.06	0.05	0.03	0.02
IPI00321190	Sulfated glycoprotein 1	61381/5.07	8					28.0	18.0	5.3	35.8	1.58	1.49	1.58	5.50	4.82	5.42	1.02
IPI00136556	Transcobalamin-2	47555/5.90	8					7.1	4.4	12.7	13.0	0.51	0.81	1.00	0.10	0.14	0.19	0.06
IPI00127560	Transthyretin	15766/5.77	8					12.7	14.9	10.7	9.6	0.42	0.60	0.71	0.09	0.12	0.16	0.07
IPI00129102	Urokinase-type plasminogen activator	48236/8.53	21					11.7	15.7	24.2	20.7	1.78	1.44	0.97	3.18	2.39	1.72	0.53
IPI00126184	Vitamin D-binding protein	53565/5.39	19					16.0	20.5	8.9	14.7	1.25	1.62	1.40	0.13	0.15	0.14	0.03

Cytoplasm protein

IPI00110850	Actin, cytoplasmic 1	41710/5.29	11					13.0	18.6	5.1	17.1	1.70	2.13	1.23	0.05	0.06	0.03	0.01
IPI00223757	Aldose reductase	35709/6.71	8					20.8	11.6	6.9	8.8	1.78	2.08	2.86	0.12	0.13	0.20	0.02
IPI00462072	Alpha-enolase	47111/6.37	7					47.1	12.2	16.5	6.1	1.26	1.09	1.31	0.02	0.01	0.02	0.00
IPI00230320	Carbonic anhydrase 1	28303/6.44	5					50.4	1.7	60.3	ND	ND	ND	ND	0.08	0.11	0.03	ND
IPI00275232	Matrix metalloproteinase 7	30009/6.79	6					5.7	12.2	7.2	11.1	3.74	5.75	5.55	0.09	0.13	0.14	0.01
IPI00121788	Peroxiredoxin-1	22162/8.26	9					14.7	8.2	7.0	19.9	1.08	1.09	1.62	0.02	0.02	0.03	0.01

Lysosome protein

IPI00111013	Cathepsin D	44925/6.71	6					12.7	10.7	15.8	12.7	0.76	0.60	1.26	0.02	0.02	0.04	0.01
IPI00128154	Cathepsin L1	37523/6.37	7					8.6	7.8	15.8	17.7	1.20	3.02	2.31	0.07	0.17	0.14	0.02
IPI00311305	Deoxyribonuclease-2-beta	40768/9.49	16					9.6	11.4	25.8	20.8	0.72	0.68	0.49	0.07	0.06	0.05	0.03
IPI00381303	Lysosomal alpha-mannosidase	114532/8.30	24					11.4	11.2	7.0	19.7	0.80	0.73	0.84	0.51	0.44	0.53	0.19

Endoplasmic Reticulum protein

IPI00319992	78 kDa glucose-regulated protein	72377/5.07	13					28.5	25.1	29.8	9.8	2.93	3.45	1.48	0.04	0.05	0.02	0.00
IPI00187353	ERO1-like protein beta	53484/8.39	13					11.6	10.4	26.3	8.0	0.38	0.44	0.23	0.01	0.01	0.01	0.01
IPI00138342	Liver carboxylesterase N	61133/5.10	8					6.6	14.4	15.1	12.2	0.30	0.45	0.29	0.06	0.08	0.05	0.06
IPI00135686	Peptidylprolyl isomerase B	23699/9.56	14					10.2	5.9	7.2	13.0	1.74	2.28	1.14	0.74	0.91	0.48	0.13

Membrane protein

IPI00553419	Desmoplakin	332706/6.43	10					18.3	16.4	9.6	13.2	1.29	1.20	1.03	0.76	0.66	0.60	0.17
IPI00881007	Endothelial chloride channel	99794/6.72	5					16.6	10.1	27.9	12.4	4.21	4.83	2.52	0.02	0.02	0.01	0.00