Table 1: Label-free quantitative analysis of mouse SVS proteome.

Accession numberUnique Peptide (A) protein  aboudance (B) average CV of peptides (%) (C) relative expression  ratio (D) percentage  of content(E) Western blot assay
ProteinMass/pIHpAdAcNHpAdAcNHp/NAd/NAc/NHpAdAcN

Seminal vesicle protein

IPI00408931Carcinoembryonic antigen-related cell adhesion molecule 1029455/6.8424 12.44.34.210.00.57 0.50 0.63 2.502.062.721.30
IPI00115065Glia-derived nexin44179/9.8544 11.77.712.517.61.76 2.18 2.00 0.420.490.470.07
IPI00469211Sulfhydryl oxidase 1, isoform 363296/8.1753 7.23.63.814.31.00 1.45 1.74 0.510.690.880.15
IPI00347009Secreted seminal-
vesicle Ly-6 protein 1
11147/8.727 31.16.311.522.61.10 1.61 0.93 0.150.210.130.04
IPI00133448Seminal vesicle antigen18266/5.5726 12.08.15.619.70.40 0.60 0.29 2.974.082.132.17
IPI00227548Seminal vesicle secretory protein 193470/8.9862 9.14.25.715.83.02 3.41 4.42 0.981.031.430.10
IPI00331164Seminal vesicle secretory protein 240824/9.89203 6.73.64.310.90.12 0.12 0.09 32.9531.0626.6783.74
IPI00227543Seminal vesicle secretory protein 3 beta29983/9.3742 9.67.93.913.12.03 2.24 2.71 3.413.514.510.49
IPI00314341Seminal vesicle secretory protein 3a29948/9.397 28.718.113.012.91.60 2.21 3.03 0.120.150.220.02
IPI00134286Seminal vesicle secretory protein 412507/8.0418 7.47.43.912.51.08 0.93 1.34 1.431.151.760.39
IPI00135709Seminal vesicle secretory protein 513011/9.5222 8.78.54.814.20.68 0.60 0.36 1.231.010.650.53
IPI00133431Seminal vesicle secretory protein 611475/5.9315 13.48.75.812.01.38 1.35 1.05 0.200.180.150.04
IPI00877305Seminal vesicle secretory protein 711060/8.9321 6.29.56.914.20.70 0.70 0.45 1.060.990.670.45
IPI00229083Serine protease inhibitor kazal-like protein10535/7.556 13.211.912.714.50.81 0.81 0.51 0.140.130.090.05
IPI00132416Serine protease inhibitor Kazal-type 38482/8.267 4.56.37.07.70.59 0.73 0.48 0.570.670.460.29
IPI00308892similar to lysozyme C, type M17039/9.7017 17.332.921.112.10.78 0.25 0.13 0.430.130.070.16

Serum protein and Secreted protein

IPI00131695Albumin68648/5.75109 12.44.64.612.13.74 2.86 1.62 8.345.943.570.66
IPI00129755Alpha-1-antitrypsin 1-245946/5.328 6.66.35.527.50.58 0.57 0.24 0.080.070.030.04
IPI00123924Alpha-1-antitrypsin 1-445969/5.2424 14.96.77.116.41.20 1.54 0.91 0.871.050.650.21
IPI00123927Alpha-1-antitrypsin 1-545862/5.449 25.54.727.110.60.27 0.71 0.72 0.080.200.220.09
IPI00117857Alpha-1-antitrypsin 1-645794/5.2510 7.97.55.843.64.32 3.51 1.67 0.150.110.060.01
IPI00128249Alpha-2-HS-glycoprotein37302/6.049 6.016.79.013.90.61 0.66 0.63 0.080.080.080.04
IPI00624663Alpha-2-macroglobulin167177/6.3811 15.27.16.215.81.01 1.27 1.53 3.794.435.691.11
IPI00169625Antiacid phosphatase variable light chain 1816955/8.505 NDNDND0.0NDNDNDNDNDND0.00
IPI00136642Antithrombin-III51971/6.1013 32.09.23.012.31.26 2.10 4.35 0.030.050.120.01
IPI00877236Apolipoprotein A-I30569/5.6437 15.410.29.520.43.80 5.04 9.13 0.410.510.970.03
IPI00377351Apolipoprotein A-IV45001/5.4123 16.717.619.820.71.10 1.09 1.36 2.942.693.590.78
IPI00322463Beta-2-glycoprotein 138593/8.599 29.55.68.236.414.67 7.08 9.01 0.060.030.040.00
IPI00331286Beta-2-microglobulin13770/8.5515 7.56.46.010.00.88 1.21 0.92 0.881.130.920.30
IPI00320420Clusterin51623/5.4647 23.811.010.328.04.38 2.45 4.81 1.941.012.110.13
IPI00323624Complement C3, isoform Long (Fragment)186538/6.2953 18.49.19.325.81.49 1.70 1.75 1.451.551.690.29
IPI00130010Complement component factor H141179/6.8522 14.819.15.015.11.26 1.54 0.53 1.201.370.500.28
IPI00114065Complement factor B (Fragment)84951/7.189 14.46.44.814.22.25 2.27 2.60 0.160.150.180.02
IPI00123744Cystatin-C15521/9.1810 8.79.96.133.92.26 2.41 1.94 0.110.110.090.01
IPI00331487Dickkopf-like
protein 1
26623/7.986 14.38.123.78.78.46 8.77 4.16 0.030.030.010.00
IPI00115522Fibrinogen, alpha61288/7.1613 7.212.99.212.31.06 1.27 2.71 0.280.310.700.08
IPI00279079Fibrinogen, beta54718/6.689 22.618.522.813.022.09 18.40 15.67 0.900.700.630.01
IPI00122312Fibrinogen, gamma50317/5.3816 14.25.09.46.92.67 2.28 1.60 0.070.050.040.01
IPI00113539Fibronectin272319/5.3911 8.01.112.710.60.16 0.29 0.20 0.050.080.060.09
IPI00117167Gelsolin, isoform 185888/5.835 33.622.822.417.93.84 3.05 3.39 0.160.120.140.01
IPI00124640Granulin64972/6.7017 10.53.615.618.61.70 1.92 2.25 0.200.210.260.03
IPI00409148Haptoglobin38727/5.887 2.88.710.713.51.32 1.54 1.63 0.030.040.040.01
IPI00469114Hemoglobin, alpha15076/7.967 7.46.96.124.02.15 2.79 6.49 3.073.709.150.42
IPI00110658Hemoglobin, beta15193/8.965 2.815.07.932.41.92 4.51 6.60 2.004.366.780.31
IPI00128484Hemopexin51308/7.9227 19.511.53.07.82.87 11.10 12.23 0.220.800.940.02
IPI00322304Histidine-rich glycoprotein HRG60401/7.2611 6.44.45.216.70.34 0.77 0.37 1.543.281.671.34
IPI00114958Isoform HMW of Kininogen-173056/6.0511 14.116.49.719.82.79 3.25 1.83 2.632.841.700.28
IPI00317340Lactotransferrin77788/8.8635 26.421.318.618.61.46 1.14 0.84 2.701.971.540.55
IPI00114403Metalloproteinase inhibitor 122613/9.145 13.44.46.626.00.72 0.69 0.79 0.240.220.260.10
IPI00123223Murinoglobulin-1165193/6.0019 5.210.53.918.90.92 1.43 1.57 0.440.640.740.14
IPI00115941Neutrophil gelatinase-associated lipocalin22861/8.9616 4.85.04.814.61.65 3.03 2.72 0.230.390.370.04
IPI00309704Nucleobindin-250273/5.0524 9.614.16.816.21.34 5.78 2.83 0.070.270.140.01
IPI00322936Plasminogen90723/6.2116 6.24.54.314.31.39 1.50 1.13 0.030.030.020.01
IPI00135156Protein FAM3B26135/8.9711 19.914.07.013.82.04 1.46 1.62 0.080.050.060.01
IPI00311104Semaphorin-3C85206/8.865 41.727.132.450.44.82 2.63 4.91 0.030.020.030.00
IPI00131830Serine protease inhibitor A3K46850/5.0519 13.58.24.718.016.28 16.63 7.69 0.110.110.050.00
IPI00139788Serotransferrin76674/6.9490 13.67.613.716.32.99 2.62 2.20 1.501.221.090.15
IPI00309214Serum amyloid P-component26230/5.985 47.536.324.66.41.17 1.08 0.65 0.060.050.030.02
IPI00321190Sulfated
glycoprotein 1
61381/5.078 28.018.05.335.81.58 1.49 1.58 5.504.825.421.02
IPI00136556Transcobalamin-247555/5.908 7.14.412.713.00.51 0.81 1.00 0.100.140.190.06
IPI00127560Transthyretin15766/5.778 12.714.910.79.60.42 0.60 0.71 0.090.120.160.07
IPI00129102Urokinase-type plasminogen activator48236/8.5321 11.715.724.220.71.78 1.44 0.97 3.182.391.720.53
IPI00126184Vitamin D-binding protein53565/5.3919 16.020.58.914.71.25 1.62 1.40 0.130.150.140.03

Cytoplasm protein

IPI00110850Actin, cytoplasmic 141710/5.2911 13.018.65.117.11.70 2.13 1.23 0.050.060.030.01
IPI00223757Aldose reductase35709/6.718 20.811.66.98.81.78 2.08 2.86 0.120.130.200.02
IPI00462072Alpha-enolase47111/6.377 47.112.216.56.11.26 1.09 1.31 0.020.010.020.00
IPI00230320Carbonic anhydrase 128303/6.445 50.41.760.3NDNDNDND0.080.110.03ND
IPI00275232Matrix metalloproteinase 730009/6.796 5.712.27.211.13.74 5.75 5.55 0.090.130.140.01
IPI00121788Peroxiredoxin-122162/8.269 14.78.27.019.91.08 1.09 1.62 0.020.020.030.01

Lysosome protein

IPI00111013Cathepsin D44925/6.716 12.710.715.812.70.76 0.60 1.26 0.020.020.040.01
IPI00128154Cathepsin L137523/6.377 8.67.815.817.71.20 3.02 2.31 0.070.170.140.02
IPI00311305Deoxyribonuclease-2-beta40768/9.4916 9.611.425.820.80.72 0.68 0.49 0.070.060.050.03
IPI00381303Lysosomal alpha-mannosidase114532/8.3024 11.411.27.019.70.80 0.73 0.84 0.510.440.530.19

Endoplasmic Reticulum protein

IPI0031999278 kDa glucose-regulated protein72377/5.0713 28.525.129.89.82.93 3.45 1.48 0.040.050.020.00
IPI00187353ERO1-like protein beta53484/8.3913 11.610.426.38.00.38 0.44 0.23 0.010.010.010.01
IPI00138342Liver carboxylesterase N61133/5.108 6.614.415.112.20.30 0.45 0.29 0.060.080.050.06
IPI00135686Peptidylprolyl isomerase B23699/9.5614 10.25.97.213.01.74 2.28 1.14 0.740.910.480.13

Membrane protein

IPI00553419Desmoplakin332706/6.4310 18.316.49.613.21.29 1.20 1.03 0.760.660.600.17
IPI00881007Endothelial chloride channel99794/6.725 16.610.127.912.44.21 4.83 2.52 0.020.020.010.00