Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes: Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating

Table 1

Proteins adsorbed on poly(MPC-co-BMA)-uncoated urine tubes.

No.	ID	M.W.^a	Score	Queries matched	pI^b
1	Tamm-Horsfall urinary glycoprotein	69,761 Da	250	15	4.96
2	Albumin	69,321 Da	353	32	5.67
3	Semenogelin-1	52,131 Da	114	6	9.26
4	Alpha-1-antichymotrypsin	47,651 Da	281	11	5.32
5	Alpha-1-antitrypsin	46,737 Da	85	3	5.37
6	Apolipoprotein A1	27,891 Da	82	4	5.27
7	IGKV1-5 protein	25,765 Da	313	7	5.74–6.30
8	Prostaglandin-H2 D-isomerase	21,029 Da	98	5	8.37
9	Apolipoprotein C3	10,846 Da	93	3	4.72
10	Protein S100-A8	10,835 Da	86	3	6.51
11	SH3 domain-binding glutamic acid-rich-like protein 3	10,438 Da	80	2	4.82

^a,bTheoretical Mr and pI, as resulted from Compute pI/Mw tool of Expasy (http://us.expasy.org/tools/pi_tool.html), are also indicated.