Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes: Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating

Table 2

Proteins which were reduced when kept in poly(MPC-co-BMA) noncoated urine tubes.

No.	ID	M.W.^a	Score	Queries matched	pI^b
1	Ceruloplasmin	122,128 Da	812	52	5.41
2	Lysosomal alpha-glucosidase	105,271 Da	152	8	5.41
3	Alpha-N-acetylglucosaminidase	82,115 Da	163	6	6.21
4	Serotransferrin	77,000 Da	1500	95	6.70
5	Alpha-1-antitrypsin	46,707 Da	253	12	5.37
6	Cell adhesion molecule 4	42,759 Da	134	6	5.59
7	Prostate-specific antigen	28,723 Da	80	2	7.26
8	IGK protein	26,218 Da	1416	4	5.74–6.30
9	IGL protein	24,777 Da	430	31	5.74–6.30
10	Alpha-1-acid glycoprotein 1	23,497 Da	239	7	5.00
11	Prostaglandin 2D synthase	22,932 Da	596	31	7.66
12	Prostaglandin-H2 D-isomerase	21,029 Da	861	42	7.66
13	Transthyretin	15,877 Da	856	37	5.35
14	Rheumatoid factor D5 light chain	12,758 Da	273	6	5.74–6.30
15	Rheumatoid factor D6 light chain	12,520 Da	273	6	5.74–6.30

^a,bTheoretical Mr and pI, as resulted from Compute pI/Mw tool of Expasy (http://us.expasy.org/tools/pi_tool.html), are also indicated.