A Comprehensive Subcellular Proteomic Survey of Salmonella Grown under Phagosome-Mimicking versus Standard Laboratory Conditions
Figure 1
Experimental workflow. A fractionation scheme based on differential centrifugation and Sarkosyl solubilization of membranes was combined with spheroplasting to obtain PERI, CYT, OM, and IM samples from . Typhimurium strain 14028. Subcellular fractions were further processed prior to high-resolution LC-MS/MS analysis.