Cathepsin D Expression in Colorectal Cancer: From Proteomic Discovery through Validation Using Western Blotting, Immunohistochemistry, and Tissue Microarrays
Table 2
Correlation of cathepsin D expression at MTB with clinico-pathological features.
Clinico-pathological parameters
No. of cases (%)1
neg/weak (%)2
Moderate (%)2
Strong (%)2
value3
Age
0.139
<65
35 (29)
14 (40)
14 (40)
7 (20)
≥65
84 (71)
50 (60)
21 (25)
13 (15)
Gender
0.586
F
59 (50)
30 (51)
17 (29)
12 (20)
M
60 (50)
34 (57)
18 (30)
8 (13)
Tumor location
0.072
Colon
82 (69)
49 (60)
19 (23)
14 (17)
Rectum
37 (31)
15 (41)
16 (43)
6 (16)
Histological type
0.177
Nonmucinous
102 (86)
52 (51)
33 (32)
17 (17)
Mucinous
16 (14)
12 (75)
2 (13)
2 (13)
Histological grade
0.211
High grade
95 (81)
47 (50)
31 (33)
17 (18)
Low grade
23 (19)
16 (70)
4 (17)
3 (13)
Vascular invasion
0.400
Negative
90 (76)
51 (57)
26 (29)
13 (14)
Positive
29 (24)
13 (45)
9 (31)
7 (24)
Perineural invasion
0.839
Negative
109 (92)
59 (54)
32 (29)
18 (17)
Positive
9 (8)
4 (44)
3 (33)
2 (22)
TNM stages
0.064
I
20 (17)
12 (60)
5 (25)
3 (15)
II
42 (36)
27 (64)
10 (24)
5 (12)
III
34 (29)
18 (53)
13 (38)
3 (9)
IV
21 (18)
7 (33)
6 (29)
8 (38)
Distant metastasis
0.038
*
No
89 (75)
52 (58)
27 (30)
10 (11)
Yes
29 (25)
12 (41)
8 (28)
9 (31)
Nodal status
0.358
Negative
66 (58)
38 (58)
16 (24)
12 (18)
Positive
47 (42)
24 (51)
17 (36)
6 (13)
Depth of invasion
0.935
T1/T2
23 (20)
13 (57)
7 (30)
3 (13)
T3/T4
93 (80)
51 (55)
27 (29)
15 (16)
5-year recurrence4
0.090
Recurrence free
58 (79)
40 (69)
14 (24)
4 (7)
Recurrence
15 (21)
6 (40)
6 (40)
3 (20)
1Percentage of the column.
2Percentage of the row.
3 value based on Pearson’s
test; *.
4Presence or absence of local or distant metachronous recurrence within 5-year followup.
*Significant correlation between expression of cathepsin D at MTB with distant metastasis.
