Human Myocardial Protein Pattern Reveals Cardiac Diseases

Table 1

Proteins identified as classifiers in coronary samples by LC-FT-ICR MS/MS. On-line measurements with electrospray ionization. Proteins identified as classifiers in aortic samples by LC-FT-ICR MS/MS. On-line measurements with electrospray ionization.

(a) FT-ICR MS/MS database search identification of classifier proteins from CHD patients

Protein ID in coronary samples	Peptide sequence	Sequence tag	m/z (charge state)	Mascot score
(P13533) Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha) MYH6_HUMAN	KLAEKDEEMEQAK	1577–1589	774.884 (2+), 516.925 (3+)	65 (92), 28
	NLQEEISDLTEQLGEGGKNVHELEKVR	1506–1532	766.896 (4+)	113
(P12883) Myosin heavy chain, cardiac muscle beta isoform (MyHC-beta) MYH7_HUMAN	KLAEKDEEMEQAK	1575–1587	774.884 (2+), 516.925 (3+)	65, 28
(P00568) Adenylate kinase isoenzyme 1 (EC 2.7.4.3) (ATP-AMP transphosphorylase) (AK1) (Myokinase) KAD1_HUMAN	GQLVPLETVLDMLR	64–77	792.447 (2+)	105
(P45379) Troponin T, cardiac muscle (TnTc) (Cardiac muscle troponin T) (cTnT) TNNT2_HUMAN	VLAIDHLNEDQLR	227–239	768.415 (2+)	98 (86)
(P62736) Actin, aortic smooth muscle (Alpha-actin-2) ACTA_HUMAN	MQKEITALAPSTMK	315–328	774.912 (2+)	18

(b) FT-ICR MS/MS database search identification of classifier proteins in AVD patients

Protein ID in aortic samples	Peptide sequence	Sequence tag	m/z (charge state)	Mascot score
(P45379) Troponin T, cardiac muscle (TnTc) (Cardiac muscle troponin T) (cTnT) TNNT2_HUMAN	DLNELQALIEAHFENR	107–122	956.482 (2+)	93
(P17661) Desmin DESM_HUMAN	FLEQQNAALAAEVNR	127–141	837.426 (2+)	90
(P02144) Myoglobin MYG_HUMAN	HPGDFGADAQGAMNK	119–133	505.894 (3+)	71 (20)
(P60709) Actin, cytoplasmic 1 (Beta-actin) ACTB_HUMAN or	IWHHTFYNELR	85–95	505.922 (3+)	70 (53) (55)
(P68032) Actin, alpha cardiac (Alpha-cardiac actin) ACTC_HUMAN	IWHHTFYNELR	87–97	758.379 (2+), 505.922 (3+)	53, 61
(P51884) Lumican precursor (Keratan sulfate proteoglycan lumican) (KSPG lumican) LUM_HUMAN	ILGPLSYSK; LKEDAVSAAFK	297–305; 171–181	489.288 (2+); 589.825 (2+)	29; 60
(P12111) Collagen alpha-3 (VI) chain precursor CO6A3_HUMAN	VAVVQYSDR	1067–1075	518.776 (2+)	59
(P12883) Myosin heavy chain, cardiac muscle beta isoform (MyHC-beta) MYH7_HUMAN	RKLEGDLK (also in MYH6_Human)	1053–1060	479.788 (2+)	21
	AQLEFNQIK	1561–1569	545.799 (2+)	25
	GSSFQTVSALHR	641–652	645.334 (2+)	25 (35) (62)
(P13533) Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha) MYH6_HUMAN	RKLEGDLK	1055–1062	479.788 (2+)	21
	AQLEFNQIK	1563–1571	545.799 (2+)	25 (26)
	GSSFQTVSALHR	643–654	645.334 (2+), 430.559 (3+)	25 (17), 47
	GKLSYTQQMEDLKR	1306–1319	566.295 (3+)	37
(P09493) Tropomyosin 1 alpha chain (Alpha-tropomyosin) TPM1_HUMAN	MEIQEIQLK	141–149	566.309 (2+)	36
(P02768) Serum albumin precursor ALBU_HUMAN	KYLYEIAR	161–168	528.299 (2+)	29
(P02511) Alpha crystallin B chain (Alpha(B)-crystallin) (Rosenthal fiber component) (Heat-shock) pro-CRYAB_HUMAN	HFSPEELK	83–90	493.751 (2+)	28
(P09669) Cytochrome c oxidase polypeptide VIc precursor (EC 1.9.3.1) COX6C_HUMAN	KAGIFQSVK	67–75	489.293 (2+)	28
(P12235) ADP/ATP translocase 1 (Adenine nucleotide translocator 1) (ANT 1) ADP, ATP carrier protein (ADT1_HUMAN)	TAVAPIER	23–30	856.487 (1+)	24 (22)
(P35555) Fibrillin-1 precursor FBN1_HUMAN	TICIETIK	843–850	489.271 (2+)	23
(P02768) Serum albumin precursor ALBU_HUMAN	KYLYEIAR	161–168	528.298 (2+)	22
(P19429) Troponin I, cardiac muscle (Cardiac troponin I) TNNI3_HUMAN	AKESLDLR	162–169	466.264 (2+)	19
(P06576) ATP synthase beta chain, mitochondrial precursor (EC 3.6.3.14) ATPB_HUMAN	FLSQPFQVAEVFTGHMGK	463–480	675.008 (3+)	16