Human Myocardial Protein Pattern Reveals Cardiac Diseases

Table 2

Proteins identified as classifiers in coronary samples by LC-MALDI-TOF/TOF mass spectrometry. Samples separated by LC deposited on plates, which are then analyzed by MALDI TOF/TOF MS. Proteins identified as classifiers in aortic samples by LC-MALDI-TOF/TOF mass spectrometry. Samples separated by LC deposited on plates, which are then analyzed by MALDI TOF/TOF MS.

(a) HPLC/MALDI TOF/TOF database search identification of classifier proteins in CHD patients

Protein ID of coronary samples	Peptide sequence	Sequence tag	m/z (charge state)	Mascot score	Protein summary score
Collagen HSCOLL NID: -Homo sapiens CAA23761 or; AF004877 NID: -Homo sapiens AAB93981	GYPGNIGPVGAAGAPGPHGPVGPAGK; 3: Hydroxyl(P) 15: Hydroxyl(P); GYPGNIGPVGAAGAPGPHGPVGPAGK; 3: Hydroxyl(P) 15: Hydroxyl(P)	327–352; 949–974	2284.147	185 (91); 91	88
(P12883) Myosin heavy chain, cardiac muscle beta isoform (MyHC-beta) MYH7_HUMAN; (Displayed; Variant CMH1-VAR_019864)	VIQYFAVIAAIGDR	191–204	1535.858.	72	88 (41.20)
(Q05639) Elongation factor 1-alpha 2 (EF-1-alpha-2); (Elongation factor 1 A-2) (eEF1A-2) (Statin S1) EF1A2_HUMAN	VETGILRPGMVVTFAPVNITTEVK	267–280	2571.421	55	74.50
Hypothetical protein DKFZp686P07163. -Homo sapiens (Human). Q5HYB7_HUMAN	SSSLLIPPLETALANFSSGPEGGVMQPVR	19–47	2954.529	80 (66)	105 (94.90)
AX885183 NID: -Homo sapiens CAE99297 or; AX885189 NID: -Homo sapiens CAE99303 or; AX885185 NID: -Homo sapiens CAE99299 or; alpha-crystallin chain B (validated); -human CYHUAB or; AF007162 NID: -Homo sapiens AAC19161 or; AX888028 NID: -Homo sapiens CAE93953	LFDQFFGEHLLESDLFPTSTSLSPFYLRPPSFLR; LFDQFFGEHLLESDXFPTSTSLSPFYLRPPSFLR	23–56; 18–51; 98–131; 23–56; 23–56	4004.027	106 (34)	133 (56.90)
(P02511) Alpha crystallin B chain (Alpha(B)-crystallin); (Rosenthal fiber component) (Heat-shock) pro CRYAB_HUMAN	LFDQFFGEHLLESDLFPTSTSL	23–44	2543.234	128	131
Crystallin, alpha B (Homo sapiens) gi∣13937813	LFDQFFGEHLLESDLFPTSTSL	23–44	2543.234	110	111
Crystallin, alpha B (Homo sapiens) gi∣4503057	LFDQFFGEHLLESDLFPTSTSL	23–44	2543.234	95	95.3
Adenylate kinase (EC 2.7.4.3) 1-human (tentative sequence) KIHUA or; AK1 protein (Adenylate kinase 1). -Homo sapiens, Q6FGX9_HUMAN or; BC001116 NID: Homo sapiens AAH01116 or; Adenylate kinase 1. -Homo sapiens (Human).; Q5T9B7_HUMAN	GQLVPLETVLDMLR	64–77; 64–77; 64–77; 79–93	1583.882	64 (49)	86.10 (69.80)
(MLRA_HUMAN) Myosin regulatory light chain 2, atrial isoform (Myosin light chain 2a) (MLC-2a) (MLC2a) (Myosin regulatory light chain 7) Myosin regulatory light Q01449	QLLLTQADKFSPAEVEQMFALTPMDLAGNIDYK	129–161	3697.849	106	131
(P45379) Troponin T, cardiac muscle; (TnTc) (Cardiac muscle troponin T) (cTnT) TNNT2_HUMAN	VLAIDHLNEDQLR	227–239	1535.81	58	85

(b) HPLC/MALDI TOF/TOF database search identification of classifier proteins in AVD patients

Protein ID of aortic samples	Peptide sequence	Sequence tag	m/z	Mascot score	Protein summary score
(P17661) Desmin DESM_HUMAN	FLEQQNAALAAEVNR	127–141	1673.860	115	133.00
mutant desmin (Homo sapiens) gi∣21358854	FLEQQNAALAAEVNR	128–142	1673.860	99 (65)	117 (84.30)
(P60709) Actin, cytoplasmic 1 (Beta-actin) ACTB_HUMAN or	IWHHTFYNELR	85–95	1515.749	82	100
(P63261) Actin, cytoplasmic 2 (Gamma-actin) ACTG_HUMAN or	IWHHTFYNELR	85–95
(P68133) Actin, alpha skeletal muscle (Alpha-actin 1) ACTS_HUMAN or	IWHHTFYNELR	85–95
(P68032) Actin, alpha cardiac (Alpha-cardiac actin) ACTC_HUMAN	IWHHTFYNELR	87–97
(P12883) Myosin heavy chain, cardiac muscle beta isoform (MyHC-beta) MYH7_HUMAN or	GSSFQTVSALHR	641–652	1289.660	79	87.30
(P13533) Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha) MYH6_HUMAN	GSSFQTVSALHR	643–654	1289.660	79	85.70
MSTP161 (Homo sapiens) gi∣33338222	SFPNLAFIR	108–116	1064.589	74	97.40
Myosin light chain 2a (Homo sapiens) gi∣10864037	SLCYIITHGDEKEE 3: Carbamidomethyl (C)	162–175	1693.774	66	122
actin-like protein (Homo sapiens) gi∣62421180	IWHHTFYNELR	2–12	1515.749	64	88.40
Myosin heavy chain alpha subunit gi∣386971	AQLEFNQIK	13–21	1090.589	60	81
alpha-1 type III collagen gi∣180413 or	GDKGETGER	7–15	948.438	57	87
unnamed protein product (Homo sapiens) gi∣1340174 or		28–36			80.1
alpha1 (III) collagen (Homo sapiens) gi∣30054 or		141–153			74.50
alpha-1 (III) collagen (Homo sapiens) gi∣930045 or		945–953			71.40
III preprocollagen alpha 1 chain (Homo sapiens) gi∣16197601		1092–1100			70.10
(P12235) ADP, ATP carrier protein, heart/skeletal muscle isoform T1 (ADP/ATP translocase 1) ADT1_HUMAN or	TAVAPIER	23–30	856.489	49	65.10
(P05141) ADP, ATP carrier protein, fibroblast isoform (ADP/ATP translocase 2) ADT2_HUMAN or	TAVAPIER	23–30	856.489	49	65.10
(P12236) ADP, ATP carrier protein, liver isoform T2 (ADP/ATP translocase 3) ADT3_HUMAN	TAVAPIER	23–30	856.489	49	65.10
Cytochrome c oxidase subunit Va, (COX5A protein). -Homo sapiens (Human). Q8TB65_HUMAN	RLNDFASTVR	98–107	1178.628	49	70.40
(P12883) Myosin heavy chain, cardiac muscle beta isoform (MyHC-beta) MYH7_HUMAN or	ILYGDFR	713–719	883.467	46	56.20
(P13533) Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha) MYH6_HUMAN	ILYGDFR	715–721	883.467	46	56.20
(P12883) Myosin heavy chain, cardiac muscle beta isoform (MyHC-beta) MYH7_HUMAN or; (P13533) Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha) MYH6_HUMAN	AVVEQTER	1689–1697; 1692–1699	931.484	38	49.20
unnamed protein product (Homo sapiens) gi∣34533821	FLLVGQTMSTLLDEDLTK	495–512	2024.062	29	47.90
Myosin, heavy polypeptide 7, cardiac muscle, beta variant (Homo sapiens) gi∣62088996 or; cardiac beta myosin heavy chain (Homo sapiens) gi∣29727	AGLLGLLEEMRDER 10: Oxidation (M)	406–419	1617.826	29	42
(O14958) Calsequestrin, cardiac muscle isoform precursor (Calsequestrin 2) CASQ2_HUMAN	EHQRPTLR	243–250	1036.565	24	41.60
alpha integrin interacting protein 63 (Homo sapiens) gi∣4468915	ESVSSFVR	27–35	910.463	25	39.60
Myosin, heavy polypeptide 7, cardiac muscle, beta variant (Homo sapiens) gi∣62088996	GSSFQTVSALHR	271–291	1289.660	47	63.60