Rapid Screening of the Epidermal Growth Factor Receptor Phosphosignaling Pathway via Microplate-Based Dot Blot Assays
Figure 6
Temporal mapping of the EGFR cascade using site-specific inhibitor molecules. The four bar graphs represent results for the following: (a) EGF stimulation, no inhibitor, and EGF stimulation in the presence of (b) neutralizing EGFR Ab, (c) GW5074—an inhibitor of Raf kinase activity, and (d) U0126—a highly selective inhibitor of MEK1/2. For each inhibitor, the arrow indicates the point of pathway inhibition. Each graph contains the time course (0–20 minutes) of expression profiles for each of the four proteins analyzed. Each bar value represents the average of three replicates.
