Research Article

Application of iTRAQ Reagents to Relatively Quantify the Reversible Redox State of Cysteine Residues

Table 1

Relative quantification of the redox state of Cys-containing tryptic peptides from yeast ADH-1 after exposure to either 1 or 5 mM H2O2. The ratio of free and reversibly oxidized thiols are compared to control levels (taken as 1.0*). 116 : 114 are the relative amounts of total thiols after H2O2 exposure. Shaded boxes are the relative amounts of reversible oxidized thiols only, referred to total thiols in control; thus, 118 : 114 and 121 : 114 are the relative amounts of reversibly oxidized thiols in controls and after exposure, respectively.

Amenable Cys tryptic peptides from yeast ADH-1Free + reversibly oxidized thiols*Reversibly oxidized thiols
Control (114 : 114)1 mM H2O2 (116 : 114)5 mM H2O2 (116 : 114)Control (118 : 114)1 mM H2O2 (121 : 114)5 mM H2O2 (121 : 114)

40YSGVCHTDLHAWHGDWPLPTK601.00
277CCSDVFNQVVK2871.00