Research Article
Application of iTRAQ Reagents to Relatively Quantify the Reversible Redox State of Cysteine Residues
Table 1
Relative quantification of the redox state of Cys-containing tryptic peptides from yeast ADH-1 after exposure to either 1 or 5 mM H2O2. The ratio of free and reversibly oxidized thiols are compared to control levels (taken as 1.0*). 116 : 114 are the relative amounts of total thiols after H2O2 exposure. Shaded boxes are the relative amounts of reversible oxidized thiols only, referred to total thiols in control; thus, 118 : 114 and 121 : 114 are the relative amounts of reversibly oxidized thiols in controls and after exposure, respectively.
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