Functional Proteomic Profiling of Phosphodiesterases Using SeraFILE Separations Platform

Table 1

Mixed-mode properties of SeraFILE surface structures^a. Table shows potential numbers of hydrogen bond donor/acceptor groups, numbers of cationic/anionic groups, and number of ring structures in the surface ligands, along with relative hydrophobicity of the ligands.

	Surface	Hydrogen bond		Cationic groups	Anionic groups	Relative hydrophobicity^b	Rings
		Donor groups	Acceptor groups
Surfaces used in the study	A	1	2		1	2
	B	1	6		3	2	1
	D	1	4		2	2	1
	M	Multipolymer				1
	N			1		1
Surfaces initially screened, but not used in the study	PN	3		3		3	1
	E			Multipolymer		3
	AP				Multipolymer	1
	AM	1	2	1		1
	S				Multipolymer	5	Multipolymer
	F			1		4
	C	1	2		1	5	1
	PL			1		3
	PA	1	1		1	4	1
	PC			1		5	1

^aIn cases of polymers, only predominant effect is considered.
^bScale 1–5: low-high.