Optimization of an Efficient Protein Extraction Protocol Compatible with Two-Dimensional Electrophoresis and Mass Spectrometry from Recalcitrant Phenolic Rich Roots of Chickpea (Cicer arietinum L.)
Table 3
Proteins identified by MALDI-TOF MS analyses.
S no.
Spot ID.
Protein identity
Peptides matched
Sequence coverage (%)
MOWSE score
Accession number (NCBI)
Mr(kDa)/pI experimental (theoretical)
Plant species
1
sp 165
NADP specific isocitrate dehydrogenase
10
17%
70
Q9XGU7_ORYSA
46.4/6.29 (46.0/6.0)
Oryza sativa
2
sp 212
Glyceraldehyde 3 phosphate dehydrogenase
9
24%
86
Q6K5G8_ORYSA
36.716/7.68 (37/6.5)
Oryza sativa
3
sp 109
Triose phosphate isomerase
6
20%
71
Q38IW8_SOYBN
27.4/5.87 (25/5.5)
Glycine max
4
sp 55
Fructokinase-like protein
9
40%
94
Q8LPE5_CICAR
26.26/5.03 (35.5, 4.5)
Cicer arietinum
5
sp 36
ATP synthase (subunit D chain)
13
36%
88
ATPQ_ARATH
19.4/5.09 (20/5.0)
Arabidopsis thaliana
6
sp 267
Porin of Pea, channel protein
2
11%
134
T12558
29.7/8.56 (30/9.5)
Phaseolus coccineus
7
sp 19
Plasma membrane intrinsic polypeptide
10
38%
74
Q9SMK5_CICAR
23.3 /4.95 (24.5/5.0)
Cicer arietinum
8
sp 248
Unidentified protein
11
35%
80
CAA06491
22.12/9.91 (44.0,9.0)
Cicer arietinum
9
sp 80
Putative pyruvate dehydrogenase E1 beta subunit isoform 1 protein
