The Application of a Three-Step Serum Proteome Analysis for the Discovery and Identification of Novel Biomarkers of Hepatocellular Carcinoma
Figure 4
Representative SDS-PAGE pattern of immunodepleted serum sample after RP-HPLC fractionation (fraction number 13). 100 μL of serum samples from seven HCC patients and five LC patients was immunodepleted and injected onto the column. Forty fractions were collected and dried, and among them 22 fractions were separated using 10–20% SDS-PAGE. Each dried fraction was dissolved in 15 μL of sample buffer and loaded onto the gel as described in the experimental procedures. Following electrophoresis, proteins were visualized by silver staining (a). For protein identification, 300 μL of serum samples was prepared again and visualized by CBB staining (b). The intensities of 14 bands were increased in all the seven HCC patients. The representative examples are indicated by arrow heads.
