An Economical High-Throughput Protocol for Multidimensional Fractionation of Proteins

<table>Exemplary analytical SDS-PAGE showing the profiles of proteins in subcellular proteome fractions extracted from a single sample of cultured human Neuro-2A cells, using the described DDF protocol. Similar to 20 <svg height="9.6750002" id="M11" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg">
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</svg>g of protein in each fraction was loaded and subsequently chemically stained using Coomassie Blue G-250. Partial differential fractionation is clearly evident.</table>

International Journal of Proteomics

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Figure 2

Figure 2: An Economical High-Throughput Protocol for Multidimensional Fractionation of Proteins 