Research Article

Proteomic and Bioinformatics Analyses of Mouse Liver Microsomes

Table 1

Proteins identified from mouse liver microsomal preparations with 2DE-based strategy.

Spot no.IPIaProtein namePredicted TMDLocation

90,91IPI00108939glyceraldehyde-3-phosphate dehydrogenase, spermatogenic0ER
6IPI00111936UDP-glucuronosyltransferase 1-2 precursor, microsomal1ER
145IPI00121833Acetyl-coenzyme A acyltransferase 10ER
102IPI00622235Transitional endoplasmic reticulum ATPase0ER
6,61,194IPI00122815Prolyl 4-hydroxylase, beta polypeptide0ER
17IPI00123176Similar to glyceraldehyde-3-phosphate dehydrogenase, 37 kDa protein0ER
134,135IPI00123342Hypoxia upregulated 11ER
2IPI00129526Endoplasmin0ER
139IPI00131459Nucleoside diphosphate kinase A0ER
179IPI00132874Splice isoform 1 of monoglyceride lipase0ER
163IPI00133522Protein disulfide-isomerase precursor0ER
49IPI00134058Thioredoxin domain containing protein 4 precursor0ER
108,145,65IPI00135284Similar to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) ISOFORM 10ER
147,148,149IPI00135686Mus musculus adult male kidney cDNA, RIKEN full-length enriched library, clone: 06100081ER
174,178,179,183IPI00135726Similar to glyceraldehyde-3-phosphate dehydrogenase (GAPDH)0ER
49,50IPI00163011Thioredoxin domain containing protein 5 precursor0ER
137IPI00226993Thioredoxin0ER
148IPI00229551ADAM41ER
62,157,158,162IPI00230108Glucose-regulated protein, full insert sequence0ER
148IPI00271869Similar to glyceraldehyde-3-phosphate dehydrogenase (GAPDH)0ER
146,147,149,150,153IPI00273646Glyceraldehyde-3-phosphate dehydrogenase0ER
187IPI00555023Glutathione S-transferase P 10ER
144IPI00319652Glutathione peroxidase0ER
84IPI0031999278 kDa glucose-regulated protein precursor0ER
153IPI00320208Elongation factor 1-beta0ER
118IPI00323357Heat shock cognate 71 kDa protein0ER
173IPI00323661Similar to glyceraldehyde-3-phosphate dehydrogenase (GAPDH)0ER
145,201IPI00462605Similar to glyceraldehyde-3-phosphate dehydrogenase (GAPDH)0ER
127IPI00469307Alpha-2-macroglobulin receptor-associated protein precursor0ER
143,152,203IPI004803432700050F09Rik protein0ER
162IPI00831714Leucine-rich repeat-containing protein 70ER (integral to membrane)
149,150IPI00352124Flavin containing monooxygenase 51ER (integral to membrane)
131IPI00132397GTP-binding protein SAR1b0ER (peripheral membrane protein)
107IPI00227657Stromal cell-derived factor 2-like protein 1 precursor0ER lumen
145IPI00123281Expressed sequence AA9597421ER membrane
7IPI00222496Protein disulfide isomerase-associated 61ER, membrane proteinb
7IPI00112322UDP-glucuronosyltransferase 2B5 precursor1ER, membrane proteinsb
156IPI00331322Microsomal glutathione S-transferase 13ER, outer membrane
151IPI00319973Membrane-associated progesterone receptor component 11ER, membrane-bound
152IPI00170316Multiple coagulation factor deficiency protein 2 homolog precursor0ER-Golgi intermediate compartment
170IPI00408892RAS-related protein RAB-70Golgi, endosomes, lysosomes
153IPI00123316Splice isoform 1 of tropomyosin 1 alpha chain0Cytoplasm
116IPI00133456Senescence marker protein-300Cytoplasm
89,129,130,151,152IPI00135085Heme-binding protein0Cytoplasm
165IPI00109061Tubulin beta-4 chain homolog0Cytoplasmic
88IPI00109073Tubulin beta-4 chain0Cytoplasmic
105,138IPI00110753Tubulin alpha-1 chain0Cytoplasmic
113,166,167,197,204IPI00110827Actin, alpha skeletal muscle0Cytoplasmic
9,129,130,151.153,166,
167,197,198
IPI00110850Actin, cytoplasmic 10Cytoplasmic
90IPI00114162Fatty acid-binding protein, epidermal0Cytoplasmic
145IPI00116277T-complex protein 1, delta subunit0Cytoplasmic
144IPI00117264DJ-1 protein0Cytoplasmic
191,164,165IPI00117348Tubulin alpha-2 chain0Cytoplasmic
137,138,164IPI00117350Tubulin alpha-4 chain0Cytoplasmic
141,153,165,132IPI00117352Tubulin beta-5 chain0Cytoplasmic
126IPI00117914Arginase 10Cytoplasmic
152IPI0012053221 kDa protein0Cytoplasmic
107,108,139,143,182IPI0012548944 KD protein (Argininosuccinate synthase)0Cytoplasmic
191IPI00626790Glutamine synthetase0Cytoplasmic
176,182,194IPI00130950Betaine-homocysteine S-methyltransferase0Cytoplasmic
99IPI00131204UDP-glucose pyrophosphorylase 20Cytoplasmic
204IPI00136929Gamma actin-like protein0Cytoplasmic
101,132IPI00169463Tubulin beta-2C Chain0Cytoplasmic
202,133IPI00221400Alcohol dehydrogenase A chain0Cytoplasmic
89IPI00221528Actin, cytoplasmic type 5 homolog0Cytoplasmic
168IPI00221890Carbonic anhydrase III0Cytoplasmic
202,133IPI00317740Guanine nucleotide-binding protein beta subunit 2-like 10Cytoplasmic
159IPI00331174T-complex protein 1, eta subunit0Cytoplasmic
154IPI00338039Tubulin, beta 20Cytoplasmic
141IPI00348094Predicted: similar to tubulin M beta 10Cytoplasmic
136IPI00404011Microtubule-associated protein0Cytoplasmic
153IPI00421223Tropomyosin alpha 4 chain0Cytoplasmic
194,195IPI00457825Similar to argininosuccinate synthase (Citrulline-aspartate ligase)0Cytoplasmic
60IPI00462072Similar to alpha enolase (2-phospho-D-glycerate hydro-lyase)0Cytoplasmic
178IPI00467066Glycine N-methyltransferase0Cytoplasmic
63,109IPI00467833Triosephosphate isomerase0Cytoplasmic
153IPI00605380Similar to tubulin alpha-2 chain (Alpha-tubulin 2)0Cytoplasmic
162IPI00123313Ubiquitin-activating enzyme E1 10Cytoplasmic and nuclear
64IPI00420745Proteasome subunit, alpha type 2, full insert sequence0Cytoplasmic and nuclear
145IPI00320165Oxidoreductase HTATIP20Cytoplasmic and nuclear
153IPI00117978Cytochrome c oxidase subunit IV isoform 1, mitochondrial precursor1Mitochondrial inner membrane
19IPI00109167NADH-ubiquinone oxidoreductase 24 kDa subunit0Mitochondrial inner membrane
158IPI00111885Ubiquinol-cytochrome-c reductase complex core protein I, mitochondrial precursor0Mitchondrial inner membrane
175IPI00121322Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor0Mitchondrial inner membrane
196IPI00128023NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial precursor0Mitchondrial inner membrane
134IPI00111908Predicted: carbamoyl-phosphate synthetase 10Mitochondrial
145IPI00114840Endonuclease G, mitochondrial precursor0Mitochondrial
70IPI003315552-oxoisovalerate dehydrogenase alpha subunit, mitochondrial precursor0Mitochondrial
94,95IPI00115607Trifunctional enzyme beta subunit, mitochondrial precursor0Mitochondrial
145IPI00115824NipSnap1 protein0Mitochondrial
22IPI00116154Cytochrome c oxidase, subunit vb, full insert sequence0Mitochondrial
15,146,147,148,149,100IPI00118986ATP synthase oligomycin sensitivity conferral protein, mitochondrial precursor0Mitochondrial
127IPI00119138Ubiquinol-cytochrome-c reductase complex core protein 2, mitochondrial precursor0Mitochondrial
147,148IPI00120984NADH-ubiquinone oxidoreductase 19 kDa subunit0Mitochondrial
137IPI00129516Ubiquinol-cytochrome c reductase complex 11 kDa protein, mitochondrial precursor0Mitochondrial
93,99,100,192,203IPI00130280ATP synthase alpha chain, mitochondrial precursor0Mitochondrial
149,150IPI00132217Tetratricopeptide repeat protein 111Mitochondrial
150,151IPI00132390NADH-ubiquinone oxidoreductase B15 subunit1Mitochondrial
101,132,137,141,153IPI00170093NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial precursor0Mitochondrial
92,93,94,95,96IPI00223092Hydroxyacyl-coenzyme A dehydrogenase/3-ketoacyl-coenzyme A0Mitochondrial
142,143,152IPI00230507ATP synthase D chain, mitochondrial0Mitochondrial
162IPI00308882NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor0Mitochondrial
149IPI0034400413 KDa differentiation-associated protein0Mitochondrial
145,146IPI00420718Hydroxymethylglutaryl-CoA synthase, mitochondrial precursor0Mitochondrial
51IPI0030888560 kDa heat shock protein, mitochondrial0Mitochondrial
153IPI00462250Similar to adenine nucleotide translocase3Mitochondrial
85,165,167,203IPI00468481ATP synthase beta chain, mitochondrial precursor0Mitochondrial
147IPI00117281Phospholipid hydroperoxide glutathione peroxidase, mitochondrial precursor0Mitochondrial and cytoplasmic
169IPI00133240Ubiquinol-cytochrome c reductase iron-sulfur subunit, mitochondrial precursor0Mitochondrial inner membrane
200,201IPI00230351Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial precursor0Mitochondrial inner membrane
174IPI00132042Pyruvate dehydrogenase E1 component beta subunit, mitochondrial precursor0Mitochondrial matrix
156IPI00315794Cytochrome b5 outer mitochondrial membrane isoform precursor1Mitochondrial outer membrane
65,109,144,170,176,100IPI00134746Argininosuccinate synthase0mitochondrion
145,146IPI00338536Succinate dehydrogenase [ubiquinone] iron-sulfur protein, mitochondrial precursor0Mitochondrion
112IPI00122547Voltage-dependent anion-selective channel protein 20Mitochondrion outer membrane
147IPI00131186Splice isoform 2 of transcription factor BTF30Nuclear
149IPI00317794Nucleolin0Nuclear
53IPI00331146UMP-CMP kinase0Nuclear
150IPI00458856Similar to ZNF91L isoform 10Nuclear
6IPI00461822E1A binding protein p3000Nuclear
55IPI00126172RIKEN cDNA 4931406C07, PTD012 homolog0Nuclear
150,151IPI0011324140S ribosomal protein S190Ribosome
104IPI00116908Similar to 40 s ribosomal protein S120Ribosome
147IPI0084979360S ribosomal protein L120Ribosome
81,127,145IPI0012597126S protease regulatory subunit S10B0Ribosome
199IPI0012360440S ribosomal protein SA0Ribosome
125IPI0013564026S protease regulatory subunit 80Ribosome
149,150,151IPI0013978060S ribosomal protein L230Ribosome
149,150IPI0022254660S ribosomal protein L220Ribosome
145IPI0031495060S acidic ribosomal protein P00Ribosome
149IPI0032256240S ribosomal protein S140Ribosome
146IPI0033109240S ribosomal protein S4, isoform0Ribosome
149,150IPI0033146160S ribosomal protein L110Ribosome
188IPI00351894Similar to ribosomal protein0Ribosome
148IPI0084979360S ribosomal protein L120Ribosome
149IPI0046588040S ribosomal protein S170Ribosome
199IPI0012360440S ribosomal protein SA0Ribosome
4,11IPI00121209Apolipoprotein A-I precursor0Secreted
149IPI00121837Ribonuclease 4 precursor1Secreted
12IPI00122429Plasma retinol-binding protein precursor0Secreted
163IPI00123920Alpha-1-antitrypsin 1–3 precursor0Secreted
163IPI00123924Alpha-1-antitrypsin 1–4 precursor0Secreted
163IPI00123927Alpha-1-antitrypsin 1–5 precursor0Secreted
162IPI00128484Hemopexin precursor1Secreted
3,117,118IPI00131695Serum albumin precursor0Secreted
98IPI00139788Serotransferrin precursor0Secreted
199IPI00323571Apolipoprotein E precursor0Secreted
135,136IPI00377351Apolipoprotein A-IV precursor0Secreted
163IPI00406302Alpha-1-antitrypsin 1-1 precursor0Secreted
100,155,156IPI0046639921 kDa protein0Secreted
156IPI00480401Major urinary protein 1 precursor0Secreted
122IPI00130661Tripeptidyl-peptidase I precursor0Secreted (lysosomal)
101IPI00115302Branched chain ketoacid dehydrogenase E1, beta polypeptide0Membrane
197IPI00120716Guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 10Membrane
21,137IPI00120719Cytochrome c oxidase, subunit va, full insert sequence0Membrane
125IPI00124790Polyposis locus protein 1-like 13Membrane
129IPI00132076Catechol O-methyltransferase1Membrane
130,142IPI00138406Ras-related protein Rap-1A0Membrane
174IPI00162780Guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 20Membrane
88,154IPI00230113Cytochrome b51Membrane
199,200IPI00353727Annexin A40Membrane
110IPI00117416Neighbor of COX40Unknown
143IPI00121271Hypothetical S-adenosyl-L-methionine-dependent methyltransferases structure containing protein0Unknown
144,108IPI00267667RIKEN cDNA 6330409N04, CLLL6 protein homolog0Unknown
101IPI00269613Eukaryotic translation initiation factor 3 subunit 20Unknown
149,150IPI0030783751 kDa protein0Unknown
203IPI00318204Sid6061p0Unknown
105IPI00273646Similar to glyceraldehyde-3-phosphate dehydrogenase0Unknown
189,190,194IPI00626790Glutamine synthetase0Unknown
50IPI0034584286 KDa PROTEIN0Unknown
51IPI0035078045 kDa protein0Unknown
133IPI0038123177 KDa protein0Unknown
144IPI00923085Probable ubiquitin-conjugating enzyme E2 FLJ25076 homolog0Unknown
146,147,150,173IPI0046029544 KDa protein0Unknown
156IPI00330913Major urinary protein 260Unknown
59IPI00467988169 kDa protein0Unknown
100,155,156IPI0046951721 kDa protein0Unknown
149IPI00130554Splice isoform 1 of SNARE-associated protein Snapin0Vesicular membrane
101,127,134IPI00131366Keratin, type II cytoskeletal 6B0Sarcolemma
83,106,107IPI00121788Peroxiredoxin 10Microbody
101,139IPI00348328Keratin Kb400Intermediate filament
156IPI00137414Left-right dynein0Cilium

aESI-Q-TOF identification, subcellular location are given for each ID number.
bThis protein is nonmembrane associated according to the annotation in the Swiss-Prot database but has one predicted TMD.