Research Article

Proteomic and Bioinformatics Analyses of Mouse Liver Microsomes

Table 2

Proteins identified from Na2CO3-extracted mouse liver microsomal membrane preparations with 1DE-based strategy.

Bands no.Accession no.Protein namePredicted TMDGRAVY scorePI valueSubcellular location

9IPI00112322UDP-glucuronosyltransferase 2B5 precursor1−0.0317.94ER
9IPI00127223UDP glucuronosyltransferase 2 family, polypeptide B361−0.0368.47ER
9IPI00222496Protein disulfide-isomerase A61−0.2925.05ER
8IPI00417182UDP-glycosyltransferase 1 family polypeptide A510.0448.33ER
9IPI00116572Cytochrome P450, family 2, subfamily d, polypeptide 92−0.0436.37ER
15IPI0011365540S ribosomal protein S60−0.91810.68ER
5IPI00129526Endoplasmin precursor (ER protein 99,94 kDa glucose-regulated protein)0−0.724.74ER
13IPI00130985Short-chain dehydrogenase CRAD200.0268.35ER
6IPI00222809Similar to GDH/6PGL endoplasmic bifunctional protein0−0.186.61ER
8IPI00230108Glucose-regulated protein, full insert sequence0−0.4795.78ER
10,11IPI00317356Paraoxonase 10−0.015.02ER
7IPI0031999278 kDa glucose-regulated protein precursor0−0.4815.07ER
13IPI00121079NADH-cytochrome b5 reductase 30−0.2038.56ER, membrane bound
9IPI00123964Cytochrome P450 2A51−0.2039.23ER, membrane bound
9IPI00114779Cytochrome P450 2C380−0.1478.69ER, membrane bound
17IPI00331322Microsomal glutathione S-transferase 130.149.67ER and mitochondrial outer membrane
17IPI00119766Cis-retinol androgen dehydrogenase 100.0059.25ER lumen
8IPI00134691UDP-glucuronosyltransferase 1-1 precursor, microsomal20.0878.87ER, integral to plasma membrane
8IPI00128287Cytochrome P450 1A21−0.2038.92ER, membrane bound
10IPI00136910Cytochrome P450 2D112−0.0096.15ER, membrane bound
9IPI00308328Cytochrome P450 2F21−0.1357.74ER, membrane bound
9,10IPI00323908Cytochrome P450 2D102−0.0736.16ER, membrane bound
7IPI00112549Long-chain-fatty-acid-CoA ligase 11−0.0456.81ER, type III membrane protein
8IPI00133522Protein disulfide-isomerase precursor0−0.3864.79ER
9IPI00116572Cytochrome P450 2D90−0.0635.93ER, membrane bound
5,6IPI00119618Calnexin precursor1−0.8754.5ER, type I membrane protein
1,10,12,14,15IPI00319973Membrane-associated progesterone receptor component 11−0.6164.57ER, membrane bound
8IPI00132475Protein ERGIC-531−0.5455.92 ER-Golgi intermediate compartment (ERGIC), type I membrane protein
8,17IPI00109061Tubulin beta-4 chain homolog0−0.4064.78Cytoplasmic
10IPI00110827Actin, alpha skeletal muscle0−0.2325.23Cytoplasmic
10,12,14IPI00110850Actin, cytoplasmic 10−0.25.29Cytoplasmic
1,2,3IPI00111908Carbamoyl-phosphate synthase0−0.126.42Cytoplasmic
1,8,9,13IPI00117348Tubulin alpha-2 chain0−0.234.94Cytoplasmic
9,10,11,12,13IPI00117914Arginase 10−0.1876.52 Cytoplasmic
17IPI00120451Fatty acid-binding protein, liver0−0.4098.59Cytoplasmic
9IPI00129028Similar to tubulin, alpha 3C isoform 10−0.2044.98Cytoplasmic
1–11,13,17IPI00130950Betaine-homocysteine S-methyltransferase0−0.368.01Cytoplasmic
1,4,6,10,11,14,15IPI00134746Argininosuccinate synthase0−0.3618.36Cytoplasmic
3,4IPI00114710Pyruvate carboxylase, mitochondrial precursor0−0.1736.25Mitochondrial
17IPI00553333Hemoglobin subunit beta-100.0927.13Mitochondrial
9IPI00134809Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex0−0.1719.1Mitochondrial
17IPI00117978Cytochrome c oxidase subunit IV isoform 1, mitochondrial precursor1−0.4129.25Mitochondrial inner membrane
15,16IPI00315794Cytochrome b5 outer mitochondrial membrane isoform precursor1−0.6024.79Mitochondrial outer membrane
13IPI00321718Prohibitin-20−2.589.83Mitochondrial, cytoplasmic, nuclear
13IPI00122547Voltage-dependent anion-selective channel protein 20−0.2237.44Outer mitochondrial Membrane
17IPI00114559Histone H2A type 10−0.57211.22Nuclear
16,17IPI00114642Histone H2B F0−0.76210.32Nuclear
8IPI00387318Cell cycle control protein 50A2−0.3318.58Membrane
15IPI00113849Splice isoform 2 of cell division control protein 42 homolog0−0.1576.16Membrane
13IPI00122549Splice isoform Pl-VDAC1 of voltage-dependent anion-selective channel protein 10−0.3348.55Membrane
15IPI00127408Ras-related C3 botulinum substrate 10−0.1018.77Membrane
15IPI00138406Ras-related protein Rap-1A0−0.3756.39Membrane
6IPI00116921Scavenger receptor class B member 120.0738.29Integral membrane protein
1IPI00121985Splice Isoform 1 of solute carrier organic anion transporter family, member 1B2120.1728.95Integral membrane protein
9IPI00124830Integrin-associated protein precursor50.5638.58Integral membrane protein
14,15IPI00131176Cytochrome c oxidase subunit 220.274.6Integral membrane protein
1IPI00132604Secretedretory carrier-associated membrane protein 340.0287.55Integral membrane protein
1IPI00135701Solute carrier organic anion transporter family, member 1A1110.2448.58Integral membrane protein
1IPI00311682Sodium/potassium-transporting ATPase alpha-1 chain precursor100.0025.3Integral membrane protein
6IPI00331214Platelet glycoprotein IV2−0.0538.61Integral membrane protein
2IPI00119063Prolow-density lipoprotein receptor-related protein 11−0.5025.17Integral to membrane
1,12,16,17IPI00124790Polyposis locus protein 1-like 130.3756.82Integral to membrane
10IPI00129677Asialoglycoprotein receptor major subunit1−0.665.99Integral to membrane
17IPI00467119Camello-like protein 110.3029.61Integral to membrane
5,8IPI00316329Keratin, type II cytoskeletal 10−0.5888.2Intermediate filament
10,11,12IPI00108844Cation-dependent mannose-6-phosphate receptor precursor1−0.1685.24Type I membrane protein
9IPI00109998H-2 class I histocompatibility antigen, D-B alpha chain precursor1−0.5086.28Type I membrane protein
4IPI00120245Integrin alpha-V precursor1−0.2465.46Type I membrane protein
3,4IPI00121190Epidermal growth factor receptor precursor2−0.3166.46Type I membrane protein
2IPI00126186Macrophage mannose receptor 1 precursor1−0.56.47Type I membrane protein
5IPI00134549Splice isoform LAMP-2A of lysosome-associated membrane glycoprotein 2 precursor1−0.0367.05Type I membrane protein
13IPI00312018Malectin1−0.2035.73Type I membrane protein
3IPI00312063Low-density lipoprotein receptor precursor1−0.3914.88Type I membrane protein
16IPI00466570Transmembrane emp24 domain-containing protein 102−0.1696.25Type I membrane protein
4IPI00108535Carcinoembryonic antigen-related cell adhesion molecule 11−0.3025.35 Type I membrane protein
5IPI00310059Polymeric-immunoglobulin receptor precursor1−0.4995.26Type I membrane protein also secreted
9IPI00121550Sodium/potassium-transporting ATPase beta-1 chain1−0.558.83Type II membrane protein
2,3IPI00134585Glutamyl aminopeptidase1−0.3445.28Type II membrane protein
10IPI00307966ADP-ribosyl cyclase 11−0.1068.64Type II membrane protein
3IPI00319509Aminopeptidase N1−0.2775.62Type II membrane protein
3IPI00458003Ectonucleotide pyrophosphatase/phosphodiesterase 31−0.3466.13Unknown
9IPI00409409CD1D1 protein1−0.1789.22Unknown
7IPI00621548NADPH-cytochrome P450 reductase1−0.4635.37Unknown
9IPI00321644Cytochrome P450 2D262−0.1056.16Unknown
1IPI00127016Hydroxysteroid 17-beta dehydrogenase 60−0.0758.63Unknown
16IPI00221721Hypothetical krab box containing protein, full insert sequence0−0.1429.84Unknown
8IPI00224073Hypothetical peptidase family M20/M25/M40 containing protein, full insert sequence0−0.015.99Unknown
16IPI00228379Ferritin light chain 20−0.4796.37Unknown
17IPI0026684217 kDa protein0−0.66810.48Unknown
15IPI00379258Similar to ferritin light chain 20−0.4548.51Unknown
3IPI00405742Plexin B20−0.35.67Unknown
2IPI00408258Structure-specific endonuclease subunit SLX40−0.7145.33Unknown
11IPI00462251Hypothetical protein LOC72792 isoform 10−0.4295.82Unknown
15IPI00605814Similar to Ferritin light chain 10−0.3586.42Unknown
10IPI00131366Keratin, type II cytoskeletal 6B0−0.4888.32Sarcolemma
10IPI00322209Keratin, type II cytoskeletal 80−0.6025.7Sarcolemma
8IPI00853991Similar to VH coding region0−0.1025.31Secreted
10IPI00126458MRNA1−0.555.66MHC class I protein complex
15IPI00121788Peroxiredoxin 10−0.2218.26Microbody